华西口腔医学杂志2024，Vol.42Issue(6)：716-722,7.DOI:10.7518/hxkq.2024.2024150
金黄地鼠不同病理状态颊囊黏膜的近红外荧光成像差异分析
Differences in near-infrared fluorescence imaging and histological analysis of cheek mucosa in golden hamsters with different pathological states
摘要
Abstract
Objective To explore and analyze the feasibility of using indocyanine green(ICG)near-infrared fluores-cence(NIF)imaging technology for the early diagnosis of oral potential malignant disorders and oral squamous cell car-cinoma.Methods 7,12-Dimethylbenz[a]anthracene in acetone solution was used to induce various pathological models of buccal mucosal lesions(mild/moderate dysplasia,severe dysplasia,squamous cell carcinoma)in golden hamster.ICG-NIF was conducted for the quantitative analysis of the fluorescence signal of lesion tissue,and evaluation of the diagnos-tic and discriminative capabilities of the ICG-NIF technology for mucosal lesions in various pathological states.Immuno-histochemical staining was perform to examine the mi-crovessel density(MVD)and microlymphatic vessel den-sity(MLVD)of mucosa in various pathological states and explore the histological reasons underlying the differ-ences in fluorescence signals.Results The results of ICG-NIF fluorescence quantitative analysis reveal the higher fluorescence intensity of mucosal lesions in the experimental group compared with that of the normal mucosa on the control side,with statistical differences(P＜0.05).Moreover,the more severe the malignancy of mucosal lesions in the experimental group,the higher the fluorescence intensity.According to histopathological analysis,the malignant pro-gression of mucosal lesions in golden hamsters was accompanied with an increase in MVD(P＜0.05)and a decrease in MLVD(P＜0.05).Conclusion The abnormal proliferation of mucosal lesions in golden hamsters exhibits a difference in ICG-NIF fluorescence signal compared with normal mucosal tissue.Fluorescence quantitative analysis methods can provide assistance in differentiation and show potential for clinical applications.关键词
吲哚菁绿/近红外荧光成像/口腔潜在恶性疾患/口腔鳞状细胞癌Key words
indocyanine green/near-infrared fluorescence imaging/oral potentially malignant disorders/oral squamous cell carcinoma分类
医药卫生引用本文复制引用
谢狄亚,单丹妮,张磊,陈盛,那潆予,王志勇..金黄地鼠不同病理状态颊囊黏膜的近红外荧光成像差异分析[J].华西口腔医学杂志,2024,42(6):716-722,7.基金项目
Key R&D Program of Jiangsu Province—Social Development Project(BE2018618) （BE2018618）
Nanjing Medical Science and Technology Development Project(YKK19089) 江苏省重点研发计划——社会发展项目(BE2018618) （YKK19089）
南京市医学科技发展项目(YKK19089) （YKK19089）
