硬壳蛤(Mercenaria mercenaria)生长性状转录组差异分析
Transcriptome analysis of growth trait differences in Mercenaria mercenaria
摘要
Abstract
The growth traits of economically important species directly affect their economic value.In this study,we focused on cohorts of the hard clam(Mercenaria mercenaria)with significant differences in growth rates.Transcriptome sequencing was performed to investigate the intergroup gene expression differences related to fast and slow growth and to explore key genes that may influence the growth rate of hard clams.The results revealed 1 590 differentially expressed genes between fast-and slow-growth groups of hard clams.Functional enrichment analysis of these differentially expressed genes indicated significant enrichment in pathways related to PPAR signal transduction,immunity,and digestion.Genes encoding protein phosphatase regulatory subunits and peroxisome proliferator-activated receptor gamma coactivator 1-alpha may affect glucose metabolism and substance transfer processes in the PPAR signaling pathway,thereby contributing to differences in growth rates among hard clams.Individuals with higher expression levels of these genes may possess stronger metabolic and substance transfer capabilities,which result in faster growth rates.In addition,genes related to lipid digestion and metabolism,such as 3-ketoacyl-CoA thiolase,were significantly downregulated in fast-growing hard clams,which suggests a slower rate of fatty acid degradation and a higher accumulation of fatty acids in these clams.Conversely,glucose-6-phosphate isomerase in pathways related to polysaccharide digestion and metabolism,and immune-related genes,such as peroxiredoxins,tissue proteases,and protein phosphatases,were significantly upregulated in fast-growing hard clams.This result indicates that fast-growing hard clam cohorts may possess stronger digestive and immune capa-bilities,thereby ensuring their faster growth rates.The findings of this study provide new insights into the genetic mechanis.关键词
硬壳蛤/生长性状/转录组/差异基因Key words
Mercenaria mercenaria/growth traits/transcriptome/differential gene分类
农业科技引用本文复制引用
梁斌,杨美洁,马兴浩,石璞,周骢,曲锦鹏,赖长苹,张涛..硬壳蛤(Mercenaria mercenaria)生长性状转录组差异分析[J].海洋科学,2024,48(9):13-23,11.基金项目
山东省重点研发计划(重大科技创新工程)项目(2023CXGC010411) （重大科技创新工程）
天津市科委项目(20YFZCSN00240) （20YFZCSN00240）
天津市农委项目(202103010) （202103010）
连云港市连云区"山海英才计划"领军人才项目 （）
连云港市花果山英才"双创计划"(创业类)项目 （创业类）
现代农业产业技术体系专项资金项目(CARS-49) （CARS-49）
广西重点研发计划项目(2021AB34014) Shandong Province Key R&D Program Project(Major Science and Technology Innovation Project),No.2023CXGC010411 （2021AB34014）
Tianjin Sci-ence and Technology Commission Project,No.20YFZCSN00240 （）
Tianjin Agricultural Committee Project,No.202103010 （）
The Leading Talent Project of the"Shanhai Talent Plan"in Lianyun District,Lianyungang City （）
Lian-yungang Huaguoshan Talent"Double Innovation Plan"(Entrepreneurship)Project （Entrepreneurship）
the Earmarked Fund for CARS,No.CARS-49 （）
The Key Research and Development Program of Guangxi Province,No.2021AB34014 （）
