海洋科学2024，Vol.48Issue(9)：24-33,10.DOI:10.11759/hykx20240130005
基于线粒体DNA比较中国、日本与美国熊本牡蛎群体遗传多样性
A comparison of the mitochondrial DNA-based genetic diver-sity of Kumamoto oyster populations from China,Japan,and the United States
摘要
Abstract
A total of 188 Kumamoto oysters were collected from six wild populations in China(Nantong,Zhejiang,Fujian,Guangdong,and Guangxi),Japan,and the United States to study the genetic diversity of cultured and wild populations.The cytochrome oxidase C subunit I(COI)and mitochondrial noncoding region(MNR)of each oyster were amplified and sequenced,and their genetic diversity and structure were analyzed.The results showed that the wild Chinese population had greater genetic diversity and more haplotype diversity in MNR sequences,whereas the Japanese and American populations had relatively lesser genetic diversity.Regarding the COI sequence,the genetic differentiation coefficient(FST)among the five Chinese populations was lower(-0.015 57-0.001 65),while the FST between the Chinese populations and the American,Japanese populations was much higher,and the FST between the American and Japanese populations was also lower(0.008 22).The results of the MNR sequences analysis were consistent,but with a large and significant genetic differentiation between the American and Japanese populations(0.109 68),indicating the great potential of MNR sequences in population genetics.AMOVA and haplotype network maps revealed genetic variations between the Chinese,American,and Japanese populations.However,a certain kinship occurred between the American and Japanese populations,which was consistent with their introduction history.The wild Chinese Kumamoto oyster population exhibited broader genetic diversity,with no genetic differ-entiation among the five populations.In contrast,the wild Japanese population is at risk of germplasm resource decline.Additionally,the cultured American population also exhibited narrow genetic diversity due to multiple generations of artificial selection and aquaculture,with obvious genetic variations from the Chinese population.This study provides a reference for the subsequent selection of breeding populations and the combination of cross-breeding populations.关键词
熊本牡蛎/COI/MNR/遗传多样性/遗传分化Key words
Kumamoto oyster/COI/MNR/genetic diversity/genetic differentiation分类
农业科技引用本文复制引用
宣益聪,常广秋,刘圣,林志华,薛清刚..基于线粒体DNA比较中国、日本与美国熊本牡蛎群体遗传多样性[J].海洋科学,2024,48(9):24-33,10.基金项目
宁波市重点研发计划(2023Z125) （2023Z125）
国家自然科学基金(32073010 （）
32002428) （）
宁波市"泛 3315"创新团队 （）
浙江万里学院基本科研业务费(SC1032080180110) Ningbo Key Research and Development Plan No.2023Z125 （SC1032080180110）
National Natural Science Foundation of China,Nos.32073010,32002428) （）
Ningbo"Pan-3315"innovation team （）
Basic Research Expenses of Zhejiang Wanli University,No.SC1032080180110 （）
