海洋科学2024，Vol.48Issue(9)：34-43,10.DOI:10.11759/hykx20240229003
球参多糖的分离纯化及体外抗凝血活性研究
Isolation,purification,and anticoagulant analysis of polysac-charides from sea cucumber Phyllophorus proteus
摘要
Abstract
In this study,two sulfated polysaccharides were isolated from Phyllophorus proteus using enzymat-ic-alkaline coupling and Sepharose DEAE Fast Flow column chromatography,and Sephadex G100 column chro-matography was used for further purification.Physicochemical property analysis indicated that the two sea cucum-ber polysaccharides were fucan sulfate(FS)and fucosylated glycosaminoglycan(FG).Two low molecular weight polysaccharides—dFS and dFG—were obtained through the degradation of FS using 10 mmol/L TFA and degrada-tion of FG via the β-elimination method,respectively.The in vitro anticoagulant assay demonstrated that FG showed good anticoagulant activity and significantly prolonged the clotting times of activated partial thromboplas-tin time(APTT),prothrombin time(PT),thrombin time(TT),and FIB.dFG significantly prolonged the clotting times of APTT,TT,and FIB but showed no significant effect on the clotting time of PT.Therefore,adjusting the molecular weight of FG can enhance the selective effects on specific coagulation pathways,thus reducing the risk of bleeding while maintaining optimal anticoagulant activities.These results indicated that FG and dFG can be de-veloped as anticoagulants.关键词
球参/岩藻聚糖硫酸酯/岩藻糖化糖胺聚糖/抗凝血Key words
Phyllophorus proteus/fucan sulfate/fucosylated glycosaminogiycan/anticoagulation分类
医药卫生引用本文复制引用
刘静雯,吴宁,耿丽华,王晶,岳洋,张全斌..球参多糖的分离纯化及体外抗凝血活性研究[J].海洋科学,2024,48(9):34-43,10.基金项目
福建省STS计划配套项目(2023T3057) Fujian Province STS Program Supporting Projects,No.2023T3057 （2023T3057）
