海洋科学2024，Vol.48Issue(9)：85-93,9.DOI:10.11759/hykx20240408001
斑马鱼早期胚胎血细胞分布特征
Distribution characteristics of blood cells in early zebrafish embryos
摘要
Abstract
The zebrafish(Danio rerio)is a model organism for investigating animal developmental biology and is used to evaluate the mechanism and related pathological characteristics of vascular and blood diseases.Fertilized eggs of zebrafish were collected and incubated until hatching to determine blood circulation in early embryos.Quantitative polymerase chain reaction(PCR)was used to analyze the expression of genes related to blood cells,blood vessels,and hemoglobin at various developmental stages.Simultaneously,o-dianisidine was used to stain embryonic hemoglobin.Following staining,an anatomical lens and microscope were used to section,examine,and photograph the embryos.Gene expression data were compared with hemoglobin distribution to analyze the devel-opment of the blood system at different stages in early zebrafish embryos.Quantitative PCR results revealed that primitive hematopoiesis began at 12 h post-fertilization,blood vessel formation began at 24 h post-fertilization,and the blood circulation system was nearly fully developed by 72 h post-fertilization.However,staining observations showed a delay;red blood cells were first detected in the yolk sac of zebrafish embryos at 30 h post-fertilization,circulating blood in the heart at 48 h post-fertilization,and red blood cells in the trunk and somites by sectioning.Red blood cells can also be found in the brain 72 h post-fertilization of embryos by sectioning.Blood circulation in zebrafish embryos matured at 96 h post-fertilization.This study details the development timeline and distribution characteristics of blood vessels and blood cells(red blood cells mainly)at gene and protein levels,providing a foundation for analyzing pathological and toxicological features of blood-related diseases using simple detection methods.关键词
斑马鱼/胚胎/血液Key words
zebrafish/embryos/blood分类
生物科学引用本文复制引用
秦思师,何秋霞,刘艳松,金瑶波,王丽娟,尤锋,谭训刚..斑马鱼早期胚胎血细胞分布特征[J].海洋科学,2024,48(9):85-93,9.基金项目
山东省自然科学基金(ZR2022MC129) （ZR2022MC129）
齐鲁工业大学(山东省科学院)科教产先导专项(2020KJC-ZD10) （山东省科学院）
国家自然科学基金(31672636) （31672636）
山东省自然科学基金联合基金(ZR2023LZL005) Shandong Natural Science Foundation,No.ZR2022MC129 （ZR2023LZL005）
Qilu University of Technology(Shandong Academy of Sciences)Science,Education and Production Pilot Project,No.2020KJC-ZD10 （Shandong Academy of Sciences）
National Natural Science Foundation of China,No.31672636 （）
Shandong Provincial Natural Science Foundation Joint Fund,No.ZR2023LZL005 （）
