海洋科学2024，Vol.48Issue(9)：94-102,9.DOI:10.11759/hykx20240228001
利用微生物法两步发酵从虾壳中提取甲壳素的绿色制备工艺研究
Green preparation of chitin from shrimp shells by two-step fermentation using microbiological method
摘要
Abstract
Shrimp shells are currently the most important source of chitin.An environmentally friendly and efficient two-step fermentation method,i.e.,demineralization(DM)and deproteinization(DP),has been employed to extract chitin from shrimp shells.In this study,an acid-producing strain(Lactiplantibacillus plantarum)and a prote-ase-producing strain(Pseudomonas aeruginosa)were isolated from different sources and used to extract chitin from shrimp shells using a two-step fermentation method under optimal conditions,followed by decolorization with 10%H2O2.The sequence of DP followed by DM demonstrated superior calcium and protein removal,achieving 96.92%±0.07%and 78.46%±1.29%,respectively.Fourier transform infrared spectroscopy,X-ray diffraction,and scanning electron microscopy were used to characterize the product,all of which indicated a high degree of structural simi-larity to commercial chitin.The crystallization index was 78.70%,and the microstructure revealed a fibrous struc-ture with pores.These results indicated that the two-step fermentation method yields chitin of higher purity.This study provides a valuable reference for the effective extraction of chitin from shrimp shells using microbiological methods.关键词
Lactiplantibacillus plantarum/Pseudomonas aeruginosa/微生物法/甲壳素提取Key words
Lactiplantibacillus plantarum/Pseudomonas aeruginosa/microbiological method/chitin extraction分类
海洋科学引用本文复制引用
周忆,杨皓月,邢荣娥,刘松,于华华,李克成,李鹏程..利用微生物法两步发酵从虾壳中提取甲壳素的绿色制备工艺研究[J].海洋科学,2024,48(9):94-102,9.基金项目
山东省重点研发计划(重大科技创新工程)项目(2022CXGC020413) （重大科技创新工程）
国家自然科学基金(42276097 （）
41976096) （）
山东省优青(ZR2022YQ37) Shandong Key R&D Plan,Major Scientific and Technologi-cal Innovation Project,No.2022CXGC020413 （ZR2022YQ37）
the National Natural Sci-ence Foundation of China,Nos.42276097,41976096 （）
the Natural Science Foundation of Shandong Province,No.ZR2022YQ37 （）
