江西农业学报2024，Vol.36Issue(11)：77-83,7.DOI:10.19386/j.cnki.jxnyxb.2024.11.011
人工种植黄精病原微生物分离鉴定及防治
Isolation,Identification and Control of Pathogenic Microorganisms from Artificially Planted Polygonatum Sibiricum
摘要
Abstract
This study used locally domesticated and cultivated susceptible Polygonatum sibiricum as the material.Tissue separation,plate streak,morphological observation and molecular biology methods were used to isolate pathogenic microorganisms and identify their species characteristics.The pathogenicity of the isolated pathogenic microorganisms to healthy tissues of Polygonatum sibiricum was verifed,and biological control methods for Polygonatum sibiricum disease were explored by 9 Chinese medicinal materials and 5 endophytic bacteria of Polygonatum.The results show that among the 10 strains of bacteria and 6 strains of fungi isolated from Polygonatum sibiricum infected tissues,3 strains of fungi BY-Z1,BY-Z3 and BY-Z4 have strong pathogenicity.After identification,they are Fusarium redolens,Fusarium solani and Minimedusa polyspora.Minimedusa polyspora is the first reported basidiomycetes isolated from Polygonatum sibiricum.Coptidis rhizoma has the strongest growth inhibition on BY-Z1,with an inhibition rate of 74.0%,followed by Angelica sinensis(28.9%).Coptidis rhizoma has the strongest growth inhibition on BY-Z3,with an inhibition rate of 66.0%,followed by mudan cortex(21.6%).Coptidis rhizoma has the strongest growth inhibition on BY-Z4,with an inhibition rate of 100.0%,followed by roots of madder(53.0%).Bacillus subtilis has inhibitory effects on pathogenic fungi BY-Z1,BY-Z3 and BY-Z4,while endophytic bacteria H3 and H4 of Polygonatum si-biricum have inhibitory effects on BY-Z1 and BY-Z4.H1 has a strong inhibitory effect on BY-Z4.关键词
黄精/病原微生物/分离鉴定/生物防治Key words
Polygonatum sibiricum/Pathogenic microorganism/Isolation and identification/Biological control分类
农业科技引用本文复制引用
王博,刘秀丽,白亚静,欧晓彬,孔新年,马婧,刘正锟..人工种植黄精病原微生物分离鉴定及防治[J].江西农业学报,2024,36(11):77-83,7.基金项目
庆阳市科技计划项目(QY2021A-F002) （QY2021A-F002）
国家自然科学基金项目(22107041) （22107041）
甘肃省高等产业支撑项目(2020C-24) （2020C-24）
甘肃省科技计划自然科学基金项目(21JR1RM328) （21JR1RM328）
陇东学院青年科技创新项目(XYZK1901) （XYZK1901）
陇东学院2024年大学生创新创业训练计划项目(202401092). （202401092）
