昆明医科大学学报2024，Vol.45Issue(11)：9-15,7.DOI:10.12259/j.issn.2095-610X.S20241102
伯氏疏螺旋体在HMC3细胞感染模型中WDR36和WRN基因的表达分析
Expression Analysis of WDR36 and WRN Genes of Borrelia Burgdorferi in HMC3 Cell Line Infection Model
摘要
Abstract
Objective The aim is to explore the molecular mechanism of neurological Lyme disease,particularly by identifying two differentially expressed genes WDR36 and WRN associated with Burkholderia infection through transcriptome analysis.Secondly,to verify their association in the pathogenesis of Borrelia burgdorferi infection and demonstrate their potential therapeutic targets.Methods By establishing a non-human primate model and utilizing high-throughput sequencing technology to obtain transcriptomic data.Through GO enrichment analysis,two differentially expressed genes with research value,WDR36 and WRN,were identified.To further validate the role of these two genes in Bb infection of Lyme disease,the HMC3 cell line of human glial cells was used as a model.The experimental group cells received Bb inoculation with different MOI(1 and 10),and cell suspensions were collected at 6 h,12 h,and 24 h after infection.Extract RNA using the triazol method and determine the relative mRNA expression levels of WDR36 and WRN genes using qPCR.Result It was found that Bb MOI=1 was the appropriate infection concentration in HMC3 cells infected with Bb.The results showed that the expression level of WDR36 was significantly upregulated at 24 h and 12 h,and the gene expression content of the 6 h PBS group increased but not significantly compared to the experimental group.The increase was significant at 12 h and 24 h,and there was a significant statistical difference with P<0.01.The expression level of WRN was significantly upregulated at 24 h and 12 h,and the gene expression content of the 6 h,12 h,and 24 h experimental groups showed an upward trend compared to their PBS group,and there was a significant statistical difference with P<0.01.Conclusion The upregulation of WDR36 and WRN may be associated with the neuropathological processes of LNB.These findings provide a new perspective for further studying the molecular mechanisms of LNB and may provide potential molecular targets for developing novel treatment strategies for Bb infection.关键词
伯氏疏螺旋体/WDR36/WRN/HMC3细胞株Key words
Borrelia burgdorferi/WDR36/WRN/HMC3 cell line分类
医药卫生引用本文复制引用
李贞,董森元,马碧书,付书雅,宝福凯,柳爱华..伯氏疏螺旋体在HMC3细胞感染模型中WDR36和WRN基因的表达分析[J].昆明医科大学学报,2024,45(11):9-15,7.基金项目
国家自然科学基金资助项目(32060180、82160304) （32060180、82160304）
云南省教育厅科学研究基金资助项目(2023J1397) （2023J1397）
