昆明医科大学学报2024，Vol.45Issue(11)：22-30,9.DOI:10.12259/j.issn.2095-610X.S20241104
miR-212-3p靶向调控NAP1L1抑制胶质瘤细胞增殖、迁移和上皮-间充质转化
miR-212-3p Targeted Regulation of NAP1L1 Inhibits Glioma Cell Proliferation,Migration and EMT
摘要
Abstract
Objective To explore the molecular mechanism of miR-212-3p in glioma cell proliferation,invasion and epithelial-mesenchymal transition(EMT).Methods The expression of miR-212-3p and NAP1L1 were detected by RT-qPCR in glioma cells.NC mimic,miR-212-3p mimic,oe-NC and oe-NAP1L1 were built and transfected in cells.CCK-8,Transwell and wound healing assay were used to evaluate the cell biological behaviour.Western blot was used to detect the expression of EMT-related biomarkers.The relationship between miR-212-3p and NAP1L1 was confirmed by the dual-luciferase reporter gene and AgO2-RIP assay.Results miR-212-3p was lowly expressed in glioma cells(P<0.0001).miR-212-3p mimic significantly inhibited the glioma cell proliferation(P<0.0001),invasion(P=0.0011)and migration(P<0.0001),and reduced the expression of EMT-related biomarkers N-cadherin(P=0.000861)and Vimentin(P=0.007430),while upregulating the expression of E-cadherin(P<0.0001).miR-212-3p targeted and negatively regulated the NAP1L1 expression.Overexpression of NAP1L1 reversed the inhibitory effects of miR-212-3p on glioma cell proliferation(P<0.0001),migration(P<0.0001),and EMT(P<0.0001).Conclusion miR-212-3p inhibits the glioma cell proliferation,migration and EMT by targeting the negative regulation of NAP1L1 expression.关键词
胶质瘤/miR-212-3p/NAP1L1/上皮-间充质转化/细胞增殖Key words
Glioma/miR-212-3p/NAP1L1/Epithelial-mesenchymal transition/Cell proliferation分类
医药卫生引用本文复制引用
郭小兵,李晓文,李恒希,曹艳,李坪..miR-212-3p靶向调控NAP1L1抑制胶质瘤细胞增殖、迁移和上皮-间充质转化[J].昆明医科大学学报,2024,45(11):22-30,9.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(202101AY070001-037) （202101AY070001-037）
