昆明医科大学学报2024，Vol.45Issue(11)：31-37,7.DOI:10.12259/j.issn.2095-610X.S20241105
喜炎平注射液对脂多糖诱导的急性肺损伤小鼠的保护作用
Protective Effects of Xiyanping Injection on Lipopolysaccharide-induced Acute Lung Injury in Mice
摘要
Abstract
Objective To explore the protective effects of Xiyanping injection against lipopolysaccharide(lipopolysaccharide,LPS)-induced acute lung injury(acute lung injury,ALI)in mice,and preliminarily investigate its mechanism.Methods The mice were randomly divided into the normal control group(NS),ALI model group(LPS),Xiyanping injection low dosage group(XYP-L,18.96 mg/kg),medium dosage group(XYP-M,37.92 mg/kg),and high dosage group(XYP-H,75.83 mg/kg)and dexamethasone positive drug group(DXM,3.03 mg/kg),with 20 in each group.Each group was injected intraperitoneally with the corresponding drug,once a day at the same time for 3 consecutive days.the NS and LPS groups were given the same 0.9%sodium chloride injection volume.Except for NS group,LPS was instilled into the trachea to induce the acute lung injury in mice 1 hour after the last administration of the above drugs.The lung tissue samples and broncho alveolar lavage fluid(BALF)samples were collected 24 hours after the intratracheal instillation of LPS.The lung coefficient was calculated.The pathological and morphological changes of lung tissue were observed macroscopically and microscopially.The total cell count in BALF was calculated under the microscope.The concentration of total protein in BALF was measured using the BCA(bicin-choninic acid)method.The content of inflammatory cytokines tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-8(IL-8)in BALF was detected using the ELISA(enzyme linked immunosorbent assay)method.The levels of malondialdehyde(MDA)and superoxide dismutase(SOD)in mouse lung tissue were measured using MDA and SOD assay kits.Results Compared with the NS group,the LPS group showed severe pathological damage in the lung tissue,increased lung coefficient,total cell count,total protein concentration,and elevated levels of TNF-α,IL-1β,and IL-8 in BALF(P<0.01).The LPS group also exhibited the higher levels of MDA in the lung tissue(P<0.001),decreased SOD activity(P<0.001).The treatment groups showed the improvements in the lung tissue damage compared to the LPS group.The treatment groups exhibited the decreased lung coefficient,total cell count,total protein concentration,TNF-α,IL-1β,and IL-8 levels in BALF(P<0.05),reduced MDA content in lung tissue(P<0.001),and increased SOD activity(P<0.05).Conclusions Xiyanping injection has a certain protective effect on LPS-induced ALI in mice,and its mechanism is related to anti-inflammatory and antioxidant effects.It can alleviate the lung inflammation through inhibiting the release of downstream inflammatory factors and oxidative stress in the lung tissue caused by LPS through antioxidant effects.关键词
喜炎平注射液/脂多糖/急性肺损伤/炎症/氧化应激Key words
Xiyanping injection/Lipopolysaccharide/Network pharmacology/Acute lung injury/Inflammation oxidative stress分类
医药卫生引用本文复制引用
聂绍燕,范苏苏,朱钰珊,彭学容,王洋,张旋..喜炎平注射液对脂多糖诱导的急性肺损伤小鼠的保护作用[J].昆明医科大学学报,2024,45(11):31-37,7.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(202101AY070001-010) （202101AY070001-010）
昆明医科大学抗炎与免疫调节药物研究科技创新团队基金资助项目(CXTD2022003) （CXTD2022003）
