昆明医科大学学报2024，Vol.45Issue(11)：46-51,6.DOI:10.12259/j.issn.2095-610X.S20241107
TLR4/NF-κB/MUC5B在过敏性鼻炎中的调控机制
Regulatory Mechanisms of TLR4/NF-κB/MUC5B in Allergic Rhinitis
摘要
Abstract
Objective To explore the regulatory mechanism of TLR4/NF-κB/MUC5B in allergic rhinitis.Methods A mouse model of allergic rhinitis was established to observe the changes in pathological characteristics of the model and to detect the expression of TLR4 and MUC5B.By reducing the expression of TLR4 by intranasal administration,the histopathological changes of TLR4,the expression of MUC5B and the concentration of inflammatory factors were observed.An in vitro cell model was established to detect the expression of MUC5B as well as to observe the changes of NF-κB and phosphorylated NF-κB(p-NF-κB)after knockdown of TLR4 by cell transfection.Results(1)TLR4 and MUC5B were highly expressed in a mouse model of allergic rhinitis(P<0.05);(2)Lowering the expression of TLR4 could reduce the expression of MUC5B and inflammatory factors,as well as alleviated allergic rhinitis(P<0.05);(3)In vitro,knockdown of TLR4 could reduce the expression of p-NF-κB and MUC5B(P<0.05).Conclusion The regulatory mechanism of TLR4/NF-κB/MUC5B in allergic rhinitis provides the new ideas for the treatment of allergic rhinitis.关键词
过敏性鼻炎/TLR4/MUC5B/NF-κBKey words
Allergic rhinitis/TLR4/MUC5B/NF-κB分类
医药卫生引用本文复制引用
王阿衡,宋镇,杨广华,何海燕..TLR4/NF-κB/MUC5B在过敏性鼻炎中的调控机制[J].昆明医科大学学报,2024,45(11):46-51,6.基金项目
青岛市科技惠民专项基金资助项目(24-2-8smjk-12-nsh) （24-2-8smjk-12-nsh）
