西南医科大学学报2024，Vol.47Issue(6)：507-511,5.DOI:10.3969/j.issn.2096-3351.2024.06.009
Lieber-DeCarli液体饲料中三种方法诱导酒精性肝病小鼠模型的比较
Comparison of three methods for inducing alcoholic liver disease in mice models using Lieber-DeCarli liquid diet
摘要
Abstract
Objective Prepare Lieber-DeCarli liquid feed and induce an alcoholic liver disease(ALD)mouse model using three methods:alcohol calorie transition,alcohol non transition,and alcohol volume transition.Compare the extent of liver function dam-age,blood lipid levels,and liver steatosis among the different groups.Method A total of 36 healthy male C57BL/6J mice were ran-domly divided into 6 groups(n=6):the alcohol calorie transition control group(A1),the alcohol calorie transition group(A2),the al-cohol non transition control group(B1),the alcohol non transition group(B2),the alcohol volume transition control group(C1),and the alcohol volume transition group(C2).Each group of mice was fed the corresponding liquid diet.On the 16th,22nd,and 28th days,mice from the corresponding groups were euthanized,and the levels of serum aspartate aminotransferase(AST),glutamic acid amino-transferase(ALT),total cholesterol(TC),triglycerides(TG),high density lipoprotein cholesterol(HDL-C),and low density lipopro-tein cholesterol(LDL-C)were measured and compared.H&E staining of the liver was used to observe pathological changes,while Oil Red O staining was used to observe hepatic steatosis.Results The serum biochemical results showed that the levels of ALT,AST,and TG in the serum of mice in each model group were higher than those in their respective control groups(P＜0.05).There was no signifi-cant difference in serum AST and ALT levels between the A2,B2,and C2 groups(P＞0.05).The serum TC and HDL-C levels in group A2 mice were higher than those in groups B2 and C2(P＜0.05),while the serum LDL-C levels in groups A2 and C2 mice were higher than those in group B2(P＜0.05).The pathological examination results showed that hepatic steatosis was observed by H&E staining,and orange-red or red lipid droplets were observed by Oil Red O staining in the liver of all model groups.Conclusion There was no dif-ference in liver function damage induced by the three methods using Lieber DeCarli liquid feed for alcoholic liver disease mouse mod-els,but different degrees of liver steatosis and lipid abnormalities could occur.关键词
Lieber-DeCarli液体饲料/酒精性脂肪肝/小鼠模型Key words
Lieber-DeCarli liquid diet/Alcoholic fatty liver disease/Mouse model分类
医药卫生引用本文复制引用
黄富春,肖国辉,汤明云,周鑫,吴旭,王天刚,李志,赵龙..Lieber-DeCarli液体饲料中三种方法诱导酒精性肝病小鼠模型的比较[J].西南医科大学学报,2024,47(6):507-511,5.基金项目
四川省科技计划项目(2022YFS0624) （2022YFS0624）
四川省中医药管理局科学技术研究专项课题(2023zd008) （2023zd008）
四川省中医药管理局科学技术研究专项课题(2023MS335) （2023MS335）
四川省保健委员会项目(川干研2024-2001) （川干研2024-2001）
四川省中医药管理局科学技术研究专项课题(2024MS219) （2024MS219）
