山东医药2024，Vol.64Issue(33)：15-18,4.DOI:10.3969/j.issn.1002-266X.2024.33.004
P300低表达食管癌细胞株TE-1的Survivivn蛋白乙酰化、增殖迁移侵袭能力变化及其机制
Changes of Survivivn protein acetylation,proliferation,migration and invasion of esophageal cancer TE-1 cell line with low expression of P300 and the mechanism
摘要
Abstract
Objective To observe the changes of Survivivn protein acetylation,proliferation,migration and inva-sion of esophageal cancer cell line TE-1 with low expression of acetyltransferase P300,and to explore the mechanism.Methods TE-1 cells in the logarithmic growth phase were divided into the si-P300 group,SI-P300-NC group and NC group,respectively.TE-1 cells in the Si-P300 group were transfected with acetyl transferase P300 down-regulated plasmid by liposome mediated method,TE-1 cells in the SI-P300-NC group were transfected with negative control plasmid by lipo-some mediated method,and TE-1 cells in the NC group were transfected without plasmid and were incubated for 48 h after transfection for follow-up experiment.Cells from each group were collected and Survivin protein acetylation level was de-tected by Co-IP assay.Cells were taken from each group and cultured for 12,24,36 and 48 h.The cell proliferation abili-ty(expressed by OD value)was detected by CCK-8.Cells in each group were selected,and the migration ability(repre-sented by cell migration distance)was detected by cell Scratch test,and the invasion ability(represented by the number of transmembrane cells)was detected by Transwell chamber test.VEGF signaling pathway-related proteins AKT1,MDM2,Bcl-2,and Caspase3 were detected by Western blotting.Results The acetylation level of Survivin protein was lower in the si-P300 group than in the si-P300-NC group and NC group(both P<0.05).The OD values of the si-P300 group were lower than those of the si-P300-NC group and NC group at 12,24,36 and 48 h after cell culture(all P<0.05).The cell migration distance was shorter and the number of transmembrane cells was smaller in the si-P300 group than in the si-P300-NC group and NC group(all P<0.05).The relative expression levels of AKT1,MDM2 and Bcl-2 protein in the si-P300 group were lower than those in the si-P300-NC group and NC group(all P<0.05),and the relative expression level of Cas-pase3 protein was higher than those in the si-P300-NC group and NC group(all P<0.05).Conclusion After transfec-tion with P300 down-regulated plasmid inhibits the expression of P300,the acetylation level of TE-1 cells decreases,and the cell proliferation,migration and invasion decrease,which may be related to the regulation of VEGF signaling pathway.关键词
乙酰化/乙酰基转移酶/P300/Survivin蛋白/食管癌/VEGF信号通路Key words
acetylation/acetyltransferase/P300/Survivin protein/esophageal carcinoma/VEGF signaling pathway引用本文复制引用
郑竞雄,孙光蕊,黄景涛,韩晓丽,赵松,杨悦,梁宗英..P300低表达食管癌细胞株TE-1的Survivivn蛋白乙酰化、增殖迁移侵袭能力变化及其机制[J].山东医药,2024,64(33):15-18,4.基金项目
河北省医学科学研究重点课题计划项目(20210985). （20210985）
