饲料研究2024，Vol.47Issue(21)：74-79,6.DOI:10.13557/j.cnki.issn1002-2813.2024.21.014
三联吡啶锌配合物对小鼠的急性毒性研究
Acute toxicity study of tripyridine-zinc complex in mice
摘要
Abstract
The experiment used Kunming mice as a vehicle to conduct an acute toxicity study on the tripyridine-zinc complex(LB-ZnI2).The absolute lethal dose(LD100)and the maximum tolerable dose(LD0)of LB-ZnI2 to Kunming mice were firstly determined by the dose declining method.Coriolis method was then used to design the animal grouping method and respective dosage.The death,clinical manifestations and pathological changes of important organs of mice were recorded during the experiment period.The results showed that,the median lethal dose(LD50)and 95％confidence limits of LB-ZnI2 provided by intraperitoneal injection were 66.0 mg/kg·bw and 53.8～80.9 mg/kg·bw.When the dosage was less than or equal to 22 mg/kg·bw,no death was happened,and there was no significant difference in the morphology,color and tissue section of the main organs between the treated mice and the normal control group.However,when the dosage was greater than 33 mg/kg·bw,the animal began to die and the number of deaths increased with the increase of drug dosage.In addition,the lung of mice were found to be congested with blood and the spleen were atrophied.Histological examination revealed decreased lymphocyte necrosis in the spleen,atrophy of the splenic corpuscle,shedding of alveolar epithelial cells in the lungs,widening of the lung interstitium,and infiltration of numerous inflammatory cells with abundant red blood cells.These changes were positively correlated with the applied dose.The study indicates that,according to the acute toxicity dosage classification of chemical substances in the Technical Guidelines for Veterinary Drug Research(2006-2011 edition),LB-ZnI2 can be judged as a moderately toxic chemical,and the drug dose should be lower than 22 mg/kg·bw in subsequent chronic toxicity and pharmacodynamics studies.关键词
三联吡啶锌金属配合物/急性毒性试验/半数致死量/小鼠Key words
tripyridine-zinc complex/acute toxicity test/median lethal dose/mice分类
农业科技引用本文复制引用
李霖珅,蒙淑玲,陈海秀,段晓鸽,罗家其,黄伟洋,江明生,陈海兰..三联吡啶锌配合物对小鼠的急性毒性研究[J].饲料研究,2024,47(21):74-79,6.基金项目
大学生创新创业训练项目(项目编号:202310593452) （项目编号:202310593452）
国家自然科学基金(项目编号:32201095) （项目编号:32201095）
巴马人才科技专项(项目编号:202100177) （项目编号:202100177）
