天津农业科学2024，Vol.30Issue(10)：33-39,49,8.DOI:10.3969/j.issn.1006-6500.2024.10.006
刀鲚MyoG基因克隆和组织表达分析
Cloning and Tissue Expression Analysis of MyoG Gene in Coilia nasus
摘要
Abstract
To explore the biological functions and tissue expression characteristics of MyoG in Coilia nasus,this study used RACE to clone the full-length cDNA sequence and conducted bioinformatics analysis.The MyoG amino acid sequences of four species,includ-ing bony fish,amphibians,birds,and mammals,were selected from GenBank.The amino acid sequences were aligned using DNA-MAN,and a phylogenetic tree was constructed using the Neighbor Joining(NJ)in MEGA 7.0.The expression of MyoG gene in different tissues of C.nasus was detected and analyzed using Quantitative Real-time PCR.The results showed that the full-length cDNA of MyoG gene in the C.nasus was 1 836 bp,including 118 bp of 5'-UTR,944 bp of 3'-UTR,and 774 bp of open reading frame(ORF),encoding a total of 257 amino acid.The MyoG amino acid sequence of the C.nasus had the typical basic helix loop helix(bHLH)do-main of MRFs,and had high homology with the MyoG amino acid sequences of other fish species,with the closest relationship being with the Monopterus albus.The relative expression level of MyoG gene in the muscle tissue of the C.nasus was the highest,while it was lower in the heart and spleen.In conclusion,this study successfully cloned the full-length cDNA sequence of MyoG gene in the C.nasus,and analyzed the bioinformatics and tissue expression characteristics of MyoG gene.The research results provides a reference for further exploring the functional characteristics of MyoG gene in C.nasus.关键词
刀鲚/MyoG基因/生物信息学分析/组织表达Key words
Coilia nasus/MyoG gene/bioinformatics analysis/organizational expression分类
农业科技引用本文复制引用
朱春月,杨培民,刘忠航,张伯序,张健,胡宗云..刀鲚MyoG基因克隆和组织表达分析[J].天津农业科学,2024,30(10):33-39,49,8.基金项目
辽宁省科技厅项目(2021JH2/10200031) （2021JH2/10200031）
辽宁省农科院项目(2020XKHBZ0706 （）
2021HQ1918) （）
辽宁省农业科学院"特色淡水鱼"学科建设专项(2024XKJS5250) （2024XKJS5250）
