畜牧与兽医2024，Vol.56Issue(12)：10-15,6.
解冻温度和时间对湖羊细管冻精解冻效果的影响
Effects of thawing temperatures and time on thawing of frozen semen straws of Hu ram
摘要
Abstract
In order to improve the quality of post-thaw Hu ram semen,this study exploredthe effects of different thawing temperatures and time on the effectiveness of semen cryopreservation.The frozen semen straws were thawed at 45,55,65,70 and 75℃for varying durations.The effects of different thawing time and temperatures on the thawing process of frozen semen straws were examined by assessing total sperm motility,progressive motility and kinematic parameters.Based on the above experiments,the impact of different thawing temperatures on the cryopreservation of semen at the optimal thawing time was determinedby assessing the parameters mentioned above,as well as the plasma membrane integrity,acrosome integrity,reactive oxygen species(ROS)levels.The results indicated that,under the condition of 45℃,the total sperm motility,progressive motility and various kinematic parameters after thawing for 20 s and 30 s were significantly(P＜0.05)higher than those in the 10 s thawing group.Under the condition of 55℃,the sperm progressive motility after thawing for 8 s was significantly(P＜0.05)higher than the 5 s thawing group.Under the conditions of 65℃and 70℃,the total sperm motility and progressive motility after tha-wing for 5 s were significantly(P＜0.05)higher than in the other groups.Under the condition of 75℃,the various kinematic parameters of sperm after thawing for 3 s were significantly(P＜0.05)higher than in the other groups.After thawing for 8 s at 55℃,the sperm plasma membrane integrity and the acrosome integrity were significantly(P＜0.05)higher than in the 37℃thawing for 30 s,70℃thawing for 5 s and 75℃thawing for 3 s groups;and the ROS level was significantly(P＜0.05)lower than in the 37℃thawing for 30 s,45℃thawing for 20 s,65℃thawing for 30 s and 75℃thawing for 3 s groups.In conclusion,thawing for 8 s at 55℃wasthe most appropriatemethod for tha-wing the frozen semen of Hu ram,which would produce ideal results in semen cryopreservation.关键词
湖羊/精液冷冻保存/解冻温度/解冻时间/精液质量Key words
Hu ram/semen cryopreservation/thawing time/semen quality分类
农业科技引用本文复制引用
张柳明,江才雨,王旭洋,孙宇轩,王健,孙晓梅,李拥军..解冻温度和时间对湖羊细管冻精解冻效果的影响[J].畜牧与兽医,2024,56(12):10-15,6.基金项目
江苏现代农业产业技术体系建设项目(JATS[2023]448) （JATS[2023]448）
