畜牧与兽医2024，Vol.56Issue(12)：16-22,7.
番鸭羽色遗传差异基因筛选及验证研究
The screening and validation of genes differentially expressed in feather color inheritance of Muscovy duck
摘要
Abstract
In order to further explore the regulation mechanism of feather color of Muscovy ducks,the experiment used black feathered and white feathered Muscovy ducks as research subjects.The transcriptome sequencing of feather follicle tissues of black and white Muscovy ducks of different feather color positions were performed by the Illumina Hiseq high throughput sequencing technique.The functions of differentially expressed genes were determined by GO enrichment analysis.RT-qPCR was used ot verify the up-regulated genes obtained initially;and the verification and screening research was conducted on feather color traits of selective breeding of Muscovy duck offspring.The results showed that the study obtained 112 significantly differentially expressed genes(63 up-regulated genes,and 49 down-regulated genes),with the ab-solute value of|log2 FC|≥6(P＜0.05)as the screening condition.The further analysis obtained 10 candidate differentially expressed genes related to the feather color trait of Muscovy ducks.The genes related to collagen myofilament production might be involved in the genet-ic regulation of black or white plumage traits of Muscovy ducks;and three up-regulated genes,MYOT,MB,NT5DC1,were initially screened here.In the expression of feather color traits of Muscovy ducks,MYOT and MB were screened as marker candidate genes for Musco-vy ducks feather color inheritance.The present research results enriched the basic research on the inheritance of plumage traits of Muscovy ducks,were of positive promotion significance,and would serve as support for research on the breeding of black feathered Muscovy ducks.关键词
番鸭/羽色遗传/上调基因/筛选与验证Key words
Muscovy duck/feather color inheritance/up-regulated gene/screening and validation分类
农业科技引用本文复制引用
陆艳凤,张蕾,孙国波,段修军,陈佳乐..番鸭羽色遗传差异基因筛选及验证研究[J].畜牧与兽医,2024,56(12):16-22,7.基金项目
泰州市种业研发攻关项目(TZZYGG202304) （TZZYGG202304）
