畜牧与兽医2024，Vol.56Issue(12)：41-49,9.
发酵乳杆菌GZHN2023LC的益生特性及其全基因组测序分析
Probiotics of Lactobacillus fermentum GZHN2023LC and its whole genome sequence analysis
摘要
Abstract
To explore the potential probiotic properties of fermented Lactobacillus isolated from Weining yellow cattle and the feasibility of developing probiotic products,we conducted the isolation and identification of the probiotics,acid and bile salt resistance tests,bacteriostatic tests and whole genome sequencing.The results showed that 11 strains of Lactobacillus fermentum were isolated from 35 samples,and the strains had good resistance to low pH and bile salts,especially Lactobacillus fermentum 5.The bacteriostatic test showed that Lactobacillus fermentum 5 had significant inhibition effect on Escherichia coli,which was named GZHN2023LC.Genome sequencing revealed that the total length of the bacterial genome was 2 077 921 bp,encoding a total of 2 011 proteins.Through comparison with databases such as Kyoto Ency-clopedia of Genes and Genomes(KEGG),Direct Homologous Clusters(COG),Gene Ontology(GO),Carbohydrate Active Enzymes(CAZy)and other databases,it was found that this genome had strong metabolic capacity of carbohydrates and amino acids,and active tran-scription and membrane transport,which might be related to the production of lactic acid by fermentation polysaccharide and the synthesis of antimicrobial peptides and extracellular polysaccharides.This study confirmed that Lactobacillus fermentum GZHN2023LC multiplied rapidly,had good resistance to acid and salt,and had significant antibacterial properties.Genome sequencing also found that this bacterium had good probiotic potential,and could be used in probiotic products.关键词
发酵乳杆菌/分离鉴定/益生特性/基因组结构/功能预测Key words
Lactobacillus fermentum/nanopore sequence/genome structure/function prediction分类
农业科技引用本文复制引用
吴道义,王明进,陈家栋,王霞,曾继晶,周礼扬,罗耀,李坤,马金萍..发酵乳杆菌GZHN2023LC的益生特性及其全基因组测序分析[J].畜牧与兽医,2024,56(12):41-49,9.基金项目
贵州省科技支撑计划项目(黔科合支撑[2021]一般153) （黔科合支撑[2021]一般153）
喀斯特山区粮改饲技术集成示范与产业化项目(黔科合服企[2021]12-03号) （黔科合服企[2021]12-03号）
农业农村部国家肉牛牦牛产业技术体系项目(CARC-37) （CARC-37）
贵州省威宁县威宁黄牛繁育基地贵州省第六批人才基地项目(黔人领发[2018]3号) （黔人领发[2018]3号）
