畜牧与兽医2024，Vol.56Issue(12)：50-56,7.
槲皮素对MRCD60纯化饲料诱导的非酒精性脂肪肝病的干预效果
Effects of quercetin on non-alcoholic fatty liver induced by MRCD60 purified feed
摘要
Abstract
To investigate the effect of quercetin(QCT)on non-alcoholic fatty liver disease(NAFLD)induced by MRCD60 purified feed,a total of 24 healthy male C57 mice with SPF grade were obtained and randomly divided into 4 groups with 6 mice in each group.The mice of the control group(Con)were fed with normal diet,and those of the model group(MRCD60)were fed with MRCD60 purified feed.Those of the QCT1 group were fed with MRCD60 purified feed and 25 mg/kg QCT.Those of the QCT2 group were fed with MRCD60 purified feed and 50 mg/kg.MRCD60 feeding for the QCT1 and QCT2 groups continued for 3 weeks and then QCT was added to the feed for another 3 weeks.After 6 weeks of experiment,the contents of ALT and AST in the serum and TC and TG in liver tissue samples collected from the mice were detected.Pathological changes in liver were observed by hematoxylin-eosin and oil red O staining.Real-time PCR was used to detect the mRNA levels of TNF-α,IL-6 and IL-1β in liver.The expression of F4/80 protein in liver was detected by immunofluorescence.The con-tent of 4-HNE in liver was detected by immunohistochemistry.The results were as follows:compared with the control group,the color changed from red to dark brown,and the liver volume,liver weight and liver index were increased in the MRCD60 group.MRCD60 feeding increased TG and TC contents in liver,serum ALT and AST activities of the organ,increased a large amount of lipid accumulation in the or-gan,increased its TNF-α,IL-6 and IL-1β mRNA levels and F4/80 protein contents.Also,MRCD60 feeding decreased GSH content in liver,but increased its MDA and 4-HNE contents.QCT supplementation at doses of 25 and 50 mg/kg significantly reduced lipid accumula-tion in liver,inhibited liver inflammation and reduced liver oxidative stress.In conclusion,QCT improved NAFLD induced by MRCD60 puri-fied feed.关键词
非酒精性脂肪肝/MRCD60/槲皮素Key words
NAFLD/MRCD60/quercetin分类
农业科技引用本文复制引用
周媛丽,曾筠雅,孟婷,石蕊,吴小马,黄克和,甘芳..槲皮素对MRCD60纯化饲料诱导的非酒精性脂肪肝病的干预效果[J].畜牧与兽医,2024,56(12):50-56,7.基金项目
江苏省产学研合作项目(BY20230700) （BY20230700）
