畜牧与兽医2024，Vol.56Issue(12)：65-73,9.
猪源荚膜血清F型多杀性巴氏杆菌的分离鉴定与致病性分析
Isolation,identification and experimental pathogenicity of capsule serogroup F Pasteurella multocida from swine
摘要
Abstract
The aim of this study was to identify a strain of Pasteurella multocida of capsule serotype F by bacterial isolation;Gram staining;biochemical identification;PCR identification of species,capsule serotype of Pasteurella multocida and main virulence factors-associated genes;and sequencing analysis,in order to provide reference for clinical diagnosis,preventive vaccine research and development,and clini-cal therapeutic drugs application.The strain was named F-HN-2301.Then,mice were used and were intra-abdominally infected with 465,48,6 CFU of the F-HN-2301 strain;and the mice all died within 24-50 hours after the infection.The virulence tests of mice showed that the F-HN-2301 strain of capsule serogroup F Pasteurella multocida exhibited higher virulence.The pathogenicity test of pigs showed that in-travenous or tracheal injection of 2.72×1010 CFU induced clinical manifestations such as elevated body temperature,mental depression,loss of appetite,and dyspnea.All of the intravenously injected pigs died in 18 to 24 hours,and 60%of the tracheal-injected pigs died in 24 to 48 hours.These results showed that the F-HN-2301 strain also had strong pathogenicity to pigs.Intravenous injection was the faster and higher mortality than tracheal injection.The results of drug sensitivity test showed that the bacterium was highly sensitive to 9 drugs such as ampicil-lin,amoxicillin and ceftiofur.These results showed that Pasteurella multocida capsule serogroup F was highly pathogenic to pigs,suggesting that pig-breeding companies and researchers should pay attention to its clinical harm and epidemic trend.关键词
多杀性巴氏杆菌/荚膜血清F型/分离鉴定/致病性Key words
Pasteurella multocida/capsule serogroup F/isolation and identification/pathogenicity分类
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张国良,吴营霞,高盛果,高志远,赵旭冉,孙哲,张云静,田克恭..猪源荚膜血清F型多杀性巴氏杆菌的分离鉴定与致病性分析[J].畜牧与兽医,2024,56(12):65-73,9.基金项目
郑洛新国家自主创新示范区创新引领型产业集群专项(201200211200) （201200211200）
