畜牧与兽医2024，Vol.56Issue(12)：97-103,7.
长链非编码RNA MSTRG.15296.5在流感病毒感染中的作用
Roles of long non-coding RNA MSTRG.15296.5 in influenza virus infection
摘要
Abstract
The aim of this study was to analyze the differentially expressed lncRNA-MSTRG.15296.5 after infection of A549 cells by influ-enza virus A/WSN/1934(WSN)of subtype H1N1,in order to provide a basis for elucidating the function and mechanism of pathogenicity-associated lncRNAs of influenza viruses.The expression of MSTRG.15296.5 was verified using qPCR;the effect of MSTRG.15296.5 on the viral replication was determined by the null plaque assay;and finally,the expression of natural immunity genes was detected and analyzed for potential target genes of MSTRG.15296.5.The results showed that the long non-coding RNA MSTRG.15296.5 promoted WSN infection and was able to down-regulate the expression of several ISGs.In conclusion,MSTRG.15296.5 promoted influenza virus replication by inhibiting the ISGs-mediated natural immune signaling pathway.This study laid experimental and theoretical basis for host-virus interactions,and sug-gested new ideas for the subsequent prevention and control of influenza virus infection.关键词
长链非编码RNA/甲型流感病毒/病毒复制/ISGsKey words
lncRNAs/IAV/virus replication/ISGs分类
农业科技引用本文复制引用
金佳昱,陈纳,梁冰,孟琪,邓璐璐,曾亦然,平继辉..长链非编码RNA MSTRG.15296.5在流感病毒感染中的作用[J].畜牧与兽医,2024,56(12):97-103,7.基金项目
国家重点研发计划项目(2021YFD1800205) （2021YFD1800205）
