Terpene extract from the stem of Celastrus orbiculatus inhibits actin cytoskeleton remodelling in gastric cancer cells by regulating the protein interaction between PTBP1 and ACTN4OA
Terpene extract from the stem of Celastrus orbiculatus inhibits actin cytoskeleton remodelling in gastric cancer cells by regulating the protein interaction between PTBP1 and ACTN4
Adjuvant chemoradiotherapy,molecular targeted therapy,and immunotherapy are frequently employed to extend the survival of patients with advanced gastric cancer(GC).However,most of these treatments have toxic side effects,drug resistance,and limited improvements in survival and quality of life.Therefore,it is crucial to discover and develop new medications targeting GC that are highly effective and have minimal toxicity.In previous studies,the total terpene extract from the stem of Celastrus orbiculatus demonstrated anti-GC activity;however,the specific mechanism was unclear.Our research utilising co-immunoprecipitation-mass spectrometry(Co-IP-MS),polypyrimidine tract binding protein 1(ptbp1)clustered regularly interspaced short palindromic repeat-associated protein 9(Cas9)-knockout(KO)mouse model,tissue microarray,and functional experiments suggests that alpha actinin-4(ACTN4)could be a significant biomarker of GC.PTBP1 influences actin cytoskeleton restructuring in GC cells by interacting with ACTN4.Celastrus orbiculatus stem extract(COE)may directly target ACTN4 and affect the interaction between PTBP1 and ACTN4,thereby exerting anti-GC effects.
Zewen Chu;Miao Zhu;Yuanyuan Luo;Yaqi Hu;Xinyi Feng;Jiacheng Shen;Haibo Wang;Masataka Sunagawa;Yanqing Liu
Institute of Translational Medicine,Medical College,Yangzhou University,Yangzhou,Jiangsu,225001,China||The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine,Yangzhou,Jiangsu,225001,ChinaDepartment of Physiology,School of Medicine,Showa University,Tokyo,142-8555,Japan
Traditional Chinese medicinePolypyrimidine tract binding protein 1Alpha actinin-4Gastric cancerActin skeleton remodelling
《药物分析学报（英文）》 2024 (008)
1158-1175 / 18
This work was financially supported by the National Natural Science Foundation of China(Grant Nos.:82274603 and 82104946),the Natural Science Foundation of Jiangsu Province,China(Grant No.:BK20210817),the Traditional Chinese Medicine Science and Technology Development Project of Jiangsu Province,China(Proj-ect code:QN202008),the Young Scientific and Technological Tal-ents Uplift Project of Jiangsu Association of Integrated Traditional Chinese and Western Medicine,China(Grant No.:JSZXTJ-2024-A05),the Postgraduate Research & Practice Innovation Program of Jiangsu Province,China(Grant No.:KYCX21_3295),and the Yangzhou University Graduate Student International Academic Exchange Special Fund Project,China.
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