基于关联分析挖掘小麦SDS沉降值相关候选基因及KASP标记开发OA北大核心CSTPCD

SDS sedimentation value is an important indicator for wheat quality breeding.In this study,genome-wide association study(GWAS)on SDS-sedimentation value was performed in 145 wheat accessions.The results revealed that a cluster of significant signals was identified at the end of 3DL,and combined with the position of the peak SNP and the distance of LD decay,the candidate interval 587.514～589.514 Mb was further determined.Then,variation sites of candidate genes within this region were explored based on the resequencing data(http://wheat.cau.edu.cn/WheatUnion/),and candidate gene association study(CGAS)was conducted in combination with SDS-sedimentation value.By integrating association results,gene annotation and gene expression analysis,TraesCS3D03G1092400 was identified as the SDS-sedimentation value-related candidate gene,and was tentatively named TaAGAP-3D.Additionally,a KASP marker Sv-3D-KASP for breeding was developed based on the polymorphic SNP of this gene,and found that TaAGAP-3 D(C)was a favorable allele for SDS-sedimentation value.Altogether,these findings provide an effective molecular marker for the genetic improvement of SDS-sedimentation value in wheat,and also provide a reference for further cloning of quality genes.