中国农业科技导报2024，Vol.26Issue(12)：18-29,12.DOI:10.13304/j.nykjdb.2024.0895
基于关联分析挖掘小麦SDS沉降值相关候选基因及KASP标记开发
Discovering of Candidate Genes for Wheat SDS-Sedimentation Value Using Association Study and Development of KASP Marker
摘要
Abstract
SDS sedimentation value is an important indicator for wheat quality breeding.In this study,genome-wide association study(GWAS)on SDS-sedimentation value was performed in 145 wheat accessions.The results revealed that a cluster of significant signals was identified at the end of 3DL,and combined with the position of the peak SNP and the distance of LD decay,the candidate interval 587.514～589.514 Mb was further determined.Then,variation sites of candidate genes within this region were explored based on the resequencing data(http://wheat.cau.edu.cn/WheatUnion/),and candidate gene association study(CGAS)was conducted in combination with SDS-sedimentation value.By integrating association results,gene annotation and gene expression analysis,TraesCS3D03G1092400 was identified as the SDS-sedimentation value-related candidate gene,and was tentatively named TaAGAP-3D.Additionally,a KASP marker Sv-3D-KASP for breeding was developed based on the polymorphic SNP of this gene,and found that TaAGAP-3 D(C)was a favorable allele for SDS-sedimentation value.Altogether,these findings provide an effective molecular marker for the genetic improvement of SDS-sedimentation value in wheat,and also provide a reference for further cloning of quality genes.关键词
小麦/SDS沉降值/全基因组关联分析/候选基因关联分析/KASP标记Key words
common wheat/SDS-sedimentation value/genome-wide association study/candidate gene association study/KASP marker分类
农业科技引用本文复制引用
刘海霞,张学勇,郝晨阳,张寅辉,庄蕾,郭梦娇,赵李,吴美娟,侯健,李甜,刘红霞..基于关联分析挖掘小麦SDS沉降值相关候选基因及KASP标记开发[J].中国农业科技导报,2024,26(12):18-29,12.基金项目
农业生物育种国家科技重大专项(2022ZD04017) （2022ZD04017）
国家重点研发计划项目(2023YFD1200404). （2023YFD1200404）
