中国农业科技导报2024,Vol.26Issue(12):63-76,14.DOI:10.13304/j.nykjdb.2023.0006
瑶药鸭脚风叶绿体基因组结构解析及密码子偏好性分析
Genomic Characteristics and Codon Preference Analysis of Schefflera octophylla(Lour.)Harms Chloroplasts
摘要
Abstract
To study the genetic structure and function,genetic codon preference,influencing factors and optimal codon of Schefflera octophylla,the Schefflera octophylla(Lour.)Harms was used as research material.The full sequence of the chloroplast genome was sequenced and assembled.The results showed that the chloroplast genome of S.octophylla was consist of a large single copy region,a small single copy region and 2 reverse repeat regions,with a total length of 156 532 bp,and 123 functional genes were obtained including 8 ribosomal rRNA genes,29 tRNA genes and 86 protein coding genes.A total of 84 SSR loci were detected.The preference analysis of codons showed that the optimal codons of S.octophylla chloroplast genomic codons tended to end in A/U bases,which indicated natural selection was main factor of preference analysis of codons.Phylogenetic analysis showed that S.actophylla had closely related to Kalopanax septemlobus.Above results provided scientific basis for the study and application of chloroplast genome of S.octophylla.关键词
鸭脚风/叶绿体基因组/密码子偏好性/最优密码子Key words
Schefflera octophylla(Lour.)Harms/chloroplast genome/codon preference/optimal code分类
农业科技引用本文复制引用
李春连,张鹏,蔡楚平,覃水凤,肖善芳,罗秋香,郭松,蓝峻峰..瑶药鸭脚风叶绿体基因组结构解析及密码子偏好性分析[J].中国农业科技导报,2024,26(12):63-76,14.基金项目
广西高校中青年教师科研基础能力提升项目(2021KY0862) (2021KY0862)
广西科技师范学院壮瑶药品质生物学重点实验室项目(GXKSKYPT2021007) (GXKSKYPT2021007)
广西科技基地和人才专项(2021AC19423). (2021AC19423)