中国农业科技导报2024，Vol.26Issue(12)：77-87,11.DOI:10.13304/j.nykjdb.2023.0151
嗜热毁丝霉木糖代谢调控机制研究
Research on Regulation Mechanism of Xylose Metabolism in Myceliophthora thermophila
摘要
Abstract
In order to improve xylose utilization efficiency of fermentation strains,the regulation mechanism of xylose metabolism in filamentous fungi Myceliophthora thermophila was investigated using genetic methods and transcriptomic techniques.The results showed that the transcription factor XlnR could positively regulate xylose metabolism and transport,as well as hemicellulase gene expression in M.thermophila,and was required for conidia germination under xylose conditions.Under xylose condition,the conidia of mutant ΔxlnR lost the ability to germinate,and the xylose utilization efficiency of mycelia was significantly decreased,while the xylose utilization efficiency was increased by 14.2%due to xlnR overexpression.Transcriptomic analysis showed that knockout of xlnR resulted in lower transcription levels of the genes involved in xylanolytic enzymes,xylose transporter,and xylose catabolic pathway.The global regulatory factor Cre-1 was an inhibitor of xylose and glucose metabolism.Disruption of cre-1 could significantly improve the utilization efficiency of xylose and glucose in M.thermophila.Meanwhile,the Cre-1 could directly inhibit the expression of xlnR and itself.The overexpression of xlnR and knockout of cre-1 made the xylose metabolic utilization efficiency increased by 100%.Above results provided a good starting strain for the establishment of the cell chassis for the efficient utilization of biomass.关键词
嗜热毁丝霉/木糖代谢/转录调控/Cre-1/XlnRKey words
Myceliophthora thermophila/xylose metabolism/transcription regulation/Cre-1/XlnR分类
农业科技引用本文复制引用
陈美欣,顾淑莹,刘佳,刘浩,李金根,田朝光..嗜热毁丝霉木糖代谢调控机制研究[J].中国农业科技导报,2024,26(12):77-87,11.基金项目
国家重点研发计划项目(2018YAF0900500). （2018YAF0900500）
