提高猪体细胞克隆胚胎发育率的研究进展OA北大核心CSTPCD

Somatic cell cloning technology can driver the somatic cells of terminally differentiated to obtain totipotency,it has the potential to develop into the living individual.The technology of porcine somatic cell cloning has a wide range of applications for the conservation and utilization of germplasm resources and the production of gene-edited pigs.However,the low rate of cloned embryo development and transfer survival limit the popularization of this technology in practical production.With the development of sequencing technology for microcell,the mechanism of abnormal development of cloned embryos has been revealed,and many researchers have tried to improve the success rate by modifying the nuclear transfer procedure or correcting the abnormal reprogramming of cloned embryos,which has achieved remarkable results.The research progress on the improvement of cloning efficiency through oocytes,donor cells and cloned embryos in recent years were summarize,which aimed at providing reference to improve the efficiency of porcine somatic cell cloning and would help to promote the application of somatic cell cloning technology in swine production.