哺乳动物精子冷冻氧化损伤以及抗氧化剂的应用研究进展OA北大核心CSTPCD

Semen cryopreservation is a crucial method for safeguarding male fertility,transgenic lines and germplasm resources of endangered species over extended periods.Cryopreserved semen can transcend the limitations of time and space,these advancements are beneficial for the rapid development of reproductive technologies such as artificial insemination and in vitro fertilization on a broad scale.However,the freeze-thawing process exerts detrimental effects on sperm,leading to its physical,chemical,and oxidative damages,which significantly reduce the quality of post-thawed sperm and affect the fertility.Among them,oxidative damage is mainly manifested as the oxygen paradox caused by the drowning of the antioxidant defense system of sperm by excessive production of reactive oxygen species(ROS)under freezing-thawing stress state.This phenomenon is considered as one of major impacts contributing to reduced survival rates and the insemination ability for post-thawed sperm.Currently,researchers are exploring the application of various antioxidants to counteract excess ROS,to balance the antioxidant defense system of frozen sperm cell.This paper mainly reviewed the recent research progress from three aspects:oxidative stress damage of sperm during the freezing process,types of antioxidants and their application status in cryopreserved sperm,so as to provide theoretical reference for further study the protective effects of antioxidants and their mechanism on frozen sperm,as well as further promoting the optimization of technical system on semen cryopreservation.