中国畜牧杂志2024,Vol.60Issue(12):58-64,7.DOI:10.19556/j.0258-7033.20240517-04
哺乳动物性别精子分选及其差异蛋白质研究进展
Research Progress on Sexed Sperm Sorting and the Differential Proteins in Mammals
摘要
Abstract
Sex sorting of mammalian spermatozoa is crucial for pre-fertilization sex control.After artificial insemination,the sexed sperm will be combined with oocyte for production of the progeny with predicted sex.In recent years,approaches of sperm sex-sorting have been discussed and optimized,and multiple methods have been obtained,including flow cytometry,Percoll® gradient centrifugation,H-Y antigen,Sephadex gel filtration,etc..However,none of them could achieve 100%accurate sperm sex-sorting.In this paper,we reviewed the research progress on the biophysical characteristics of sexed sperm,the current status of commercial sperm sex-sorting technologies,and the different proteins of sexed sperm,which will provide theoretical reference for further research and optimization of mammalian sperm sex-sorting technology and its popularization and application.关键词
X精子/Y精子/流式细胞分选术/试剂盒分离法/差异蛋白质Key words
X sperm/Y sperm/Flow cytometry sorting/Kits sorting/Differential proteins分类
农业科技引用本文复制引用
李春艳,张彦..哺乳动物性别精子分选及其差异蛋白质研究进展[J].中国畜牧杂志,2024,60(12):58-64,7.基金项目
云南省基础研究专项-青年基金项目(202201AU070224) (202201AU070224)
云南省重大科技专项计划(202102AE090039) (202102AE090039)