草业学报2024，Vol.33Issue(12)：122-133,12.DOI:10.11686/cyxb2024048
纹党连作土壤浸提液对其种子萌发及幼苗生长的自毒效应研究
A study of autotoxic effects of soil extract from continuously cropped Codonopsis pilosula var.modesta on seed germination and seedling growth
摘要
Abstract
Autotoxicity is one of the serious problems in Codonopsis pilosula var.modesta cultivation.The purpose of this study was to investigate the autotoxicity effects of soil extract from continuously cropped C.pilosula var.modesta on seed germination and seedling growth,and to screen out potential autotoxic compounds of C.pilosula var.modesta.Rhizosphere soil of C.pilosula var.modesta continuously cropped for 1,2 and 3 years was collected;extracts from the collected soil were prepared,and germination and seedling growth tests for C.pilosula var.modesta were conducted.Potentially autotoxic compounds in the rhizosphere soil extracts were identified by gas chromatography-mass spectrometry(GC-MS).It was found that the soil extract for all three continuous cropping durations had an effect of"low promotion and high inhibition"on the seed germination and seedling growth of C.pilosula var.modesta.However,the inhibition effect of continuous cropping for 3 years was the strongest,and that of continuous cropping for 1 year was the weakest.The chlorophyll content and the catalase,peroxidase and superoxide dismutase in leaves initially increased and then decreased with increase in soil extract concentration,and the contents of malondialdehyde and proline increased with increase in soil extract concentration.Again,these effects were strongest for extracts from soils after three years of continuous cropping.The GC-MS analysis detected,respectively,12,18 and 8 different potentially autotoxic compounds(including hydrocarbons,alcohols,ketones and fatty acids),in extracts from soils continuously cropped for 1,2 and 3 years.In summary,extracts from soils continuously cropped with C.pilosula var.modesta had autotoxic effects on seed germination and seedling growth.Autotoxicity is one of the causes of continuous cropping vigor loss in C.pilosula var.modesta.关键词
纹党/连作障碍/自毒作用/气质联用技术/自毒物质Key words
Codonopsis pilosula var.modesta/continuous cropping obstacles/autotoxicity/GC-MS/autotoxic substance引用本文复制引用
陈莺宇,邱亚娟,邵晓龄,黄钰芳,杨扶德,陈林杰,陈红刚,谢田朋..纹党连作土壤浸提液对其种子萌发及幼苗生长的自毒效应研究[J].草业学报,2024,33(12):122-133,12.基金项目
国家自然科学基金(82160714),甘肃省自然科学基金(21JR1RA271),国家重点研发计划(2018YFC1706305),甘肃省高等学校产业支撑引导项目(2021CYZC-40),研究生"创新之星"项目(2023CXZX-764)和敦煌医学与转化教育部重点实验室项目(DHYX20-13)资助. （82160714）
