草业学报2024，Vol.33Issue(12)：134-146,13.DOI:10.11686/cyxb2024086
草地早熟禾种带可培养细菌多样性及18个菌株促生特性研究
A study of the diversity of cultivable seed-borne bacteria in Poa pratensis and the growth-promoting characteristics of 18 bacterial strains
摘要
Abstract
This study investigated the diversity of cultivable seed-borne bacteria of Poa pratensis to identify strains with superior plant growth-promoting properties.The cultivable seed-borne bacteria of three varieties of P.pratensis were isolated by the dilution plate method and 30 bacterial isolates were obtained.Through 16S rRNA sequence analysis,18 representative isolates with different morphological characteristics were identified and phylogenetic trees were constructed.It was found that the isolated cultivable seed-borne bacteria so identified belonged to the phyla Firmicutes,Actinobacteria and Proteobacteria,and included six genera,Bacillus,Paenibacillus,Pseudomonas,Curtobacterium,Stenotrophomonas and Erwinia.Among them,Bacillus is the common genus of three varieties.The nitrogen fixation,indole-3-acetic acid-producing,phosphorus solubilization and extracellular enzyme(protease,cellulase and amylase)production of the 18 bacterial taxa were determined and it was found that strains designated BM8 and KTJ14 possessed all three of the above functions.In addition,the biofilm formation ability and swimming motility of the strains were determined by crystal violet staining and swimming medium,and the correlation between these two traits and the growth-promoting function of isolated taxa is discussed.A strain designated KTJ16 had the strongest biofilm formation ability,and the swimming motility of taxa designated SW5 and KTJ18 was significantly stronger than that of other taxa.Based on multiple characteristics,the strains with superior growth-promoting characteristics were determined to be BM8,SW5,KTJ14,KTJ16 and KTJ18.关键词
固氮/溶磷/产IAA/产胞外酶/生物被膜/泳动能力Key words
nitrogen fixation/phosphorus solubilizing/IAA-producing/producing extracellular enzymes/biofilm/swimming motility引用本文复制引用
谢金晶,朱绍玮,黄荣,杨杰,侯瑄,张振粉..草地早熟禾种带可培养细菌多样性及18个菌株促生特性研究[J].草业学报,2024,33(12):134-146,13.基金项目
国家自然科学基金(32060369),甘肃省杰出青年基金(20JR10RA562)和草业生态系统教育部重点实验室-人才培育项目(KLGE202203)资助. （32060369）
