草业学报2024，Vol.33Issue(12)：160-174,15.DOI:10.11686/cyxb2024042
基于宏基因组分析玉米连作对土壤微生物群落结构的影响
Effects of maize continuous cropping on soil microbial community structure based on metagenomic analysis
摘要
Abstract
The aim of this study was to explore the effects of maize(Zea mays)continuous cropping on soil microorganisms.Soil samples were collected from the Ganzhou District of Zhangye City from fields continuously used to cultivate maize for 5,10,15,20,and more than 21 years,and from Linze County from fields continuously used to cultivate maize for 10,15,20 and more than 21 years.The soil samples were subjected to metagenomic analyses.The results showed that the soil bacterial abundance in the two areas increased and then decreased as the number of years of continuous cropping increased,and the diversity continued to decrease with extended continuous cropping.Across the time series,the relative abundance of the dominant phyla Proteobacteria and Actinobacteria in Ganzhou District initially increased and then decreased,and had further significantly decreased at 20 years of continuous cropping(P<0.05).The relative abundance of Acidobacteria in Linze County continued to decline,and was significantly lower at 20 years of continuous cropping than at 10 years(P<0.05).With increasing number of years of continuous cropping,fungal abundance and diversity continued to increase.The relative abundance of the fungal genera Colletotrichum and Fusarium in Ganzhou District was significantly increased(P<0.05)at 15 and 20 years of continuous cropping,and that of Fusarium in Linze County fluctuated at 15 and 21 years of continuous cropping.Analyses of beta diversity revealed that the structure of soil bacterial and fungal communities differed significantly,depending on the number of years of continuous cropping.In Ganzhou District,metabolic pathways,microbial metabolism,carbon metabolism,and pyruvate metabolism functions of the microbial community were significantly lower(P<0.05)at 10 years than at 5 years of continuous cropping.In Linze County,metabolic pathways,biosynthesis of secondary metabolites,and pyruvate metabolism functions were significantly(P<0.05)higher at 20 years than at 10 years of continuous cropping.In contrast,two-component system functions were significantly(P<0.05)lower at 20 years than at 10 years of continuous cropping.The results of this study indicate that the duration of continuous cropping affects the diversity,community structure,and functions of soil microbes.The decrease in the relative abundance of Proteobacteria and Actinobacteria and the increase in the relative abundance of Colletotrichum and Fusarium in Ganzhou District,and the decrease in the relative abundance of Acidobacteria and the increase in the relative abundance of Fusarium in Linze County might contribute to maize continuous cropping decline.The results of our study provide a basis for unravelling the microbiological mechanism of continuous cropping decline.关键词
玉米/土壤连作/宏基因组/土壤微生物/群落结构Key words
maize/soil continuous cropping/metagenomic/soil microorganism/community structure引用本文复制引用
张晨阳,金梦军,许永锋,杨成德..基于宏基因组分析玉米连作对土壤微生物群落结构的影响[J].草业学报,2024,33(12):160-174,15.基金项目
国家自然科学基金(No.31660148)资助. （No.31660148）
