声门上型喉癌患者血清LncRNA MALAT1、miR-204-5p表达与预后的关系OACSTPCD
Relationship between serum LncRNA MALAT1 and miR-204-5p expression and prognosis in patients with supraglottic carcinoma
目的 探讨声门上型喉癌患者血清LncRNA MALAT1、miR-204-5p表达与预后的关系.方法 分析2016年11月～2020年11月于保定市第一中心医院耳鼻咽喉科接受治疗的118例声门上型喉癌患者作为观察组,另选取同期体检的118名健康成年人作为对照组.观察组根据生存情况分为预后不良组(n=20例)与预后良好组(n=98例).应用实时荧光定量聚合酶链反应(qRT-PCR)方法,对LncRNA MALAT1、miR-204-5p的表达量进行检测;Pearson法分析血清LncRNA MALAT1与miR-204-5p水平之间的关联性;构建受试者工作特征(ROC)曲线,评估血清LncRNA MALAT1及miR-204-5p水平对声门上型喉癌患者预后方面的预测价值.结果 观察组的血清LncRNA MALAT1水平显著高于对照组(P<0.05),miR-204-5p水平显著低于对照组(P<0.05);血清LncRNA MALAT1与miR-204-5p表达水平呈负相关(r=-0.487,P<0.001);血清中LncRNA MALAT1与miR-204-5p表达水平与肿瘤T分级、淋巴结转移及分化程度间存在显著相关性(P<0.05);相较于预后良好组,预后不良组患者患者血清中LncRNA MALAT1的浓度明显升高(P<0.05),T1级、无淋巴结转移、高分化、临床Ⅰ～Ⅱ期及血清miR-204-5p水平显著降低(P<0.05).通过ROC曲线分析,LncRNA MALAT1与miR-204-5p联合应用于预测声门上型喉癌患者预后,其曲线下面积(AUC)显著大于LncRNA MALAT1单独预测的AUC(Z=3.079,P=0.002)及miR-204-5p单独预测的AUC(Z=3.338,P=0.001).结论 声门上型喉癌患者血清LncRNA MALAT1水平升高,血清miR-204-5p水平降低,对预测声门上型喉癌患者的预后情况展现出一定价值.
OBJECTIVE To investigate the relationship between serum LncRNA MALAT1 and miR-204-5p expression and prognosis in patients with supraglottic carcinoma.METHODS From November 2016 to November 2020,118 patients with supraglottic carcinoma who were treated in the Department of Otolaryngology,Baoding First Central Hospital were analyzed,as the observation group,and 118 healthy adults were selected as the control group.The observation group was divided into poor prognosis group(n=20 cases)and good prognosis group(n=98 cases)according to the survival situation.Real-time fluorescence quantitative PCR(qRT-PCR)technology was applied to detect the expression levels of LncRNA MALAT1 and miR-204-5p.Pearson method was applied to analyze the correlation between serum LncRNA MALAT1 and miR-204-5p levels.Receiver operating characteristic(ROC)curves were plotted to analyze the predictive value of serum LncRNA MALAT1 and miR-204-5p levels for the prognosis of patients with supraglottic carcinoma.RESULTS The serum LncRNA MALAT1 level in the observation group was prominently higher than that in the control group(P<0.05),and the miR-204-5p level was prominently lower than that in the control group(P<0.05).The expression level of serum LncRNA MALAT1 was negatively correlated with miR-204-5p(r=-0.487,P<0.001).The levels of serum LncRNA MALAT1 and miR-204-5p were related to T staging,lymph node metastasis,and differentiation degree(P<0.05).The level of serum LncRNA MALAT1 in the poor prognosis group was greatly higher than that in the good prognosis group(P<0.05),while the proportions of T1 stage,no lymph node metastasis,high differentiation,clinical stages Ⅰ-Ⅱ,and the level of serum miR-204-5p were greatly lower than those in the good prognosis group(P<0.05).ROC curve showed that the AUC of LncRNA MALAT1 combined miR-204-5p in predicting the prognosis of supraglottic carcinoma patients was prominently higher than that of LncRNA MALAT1 alone(Z=3.079,P=0.002)and miR-204-5p alone(Z=3.338,P=0.001).CONCLUSION Serum LncRNA MALAT1 level is elevated and serum miR-204-5p level is reduced in patients with supraglottic carcinoma.They have certain value in evaluating the prognosis of patients with supraglottic carcinoma.
甄全胜;王立娟;刘冬梅
保定市第一中心医院耳鼻咽喉科,河北 保定 071000保定市第二医院耳鼻咽喉科,河北 保定 071000保定市第一中心医院总院门诊部,河北 保定 071000
喉肿瘤预后LncRNA MALAT1miR-204-5p
Laryngeal NeoplasmsPrognosisLncRNA MALAT1miR-204-5p
《中国耳鼻咽喉头颈外科》 2024 (11)
698-702,5
河北省2024年度医学科学研究课题计划(20242031)
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