基质金属蛋白酶抑制因子3和纤维连接蛋白1在鼻咽癌组织中的表达及临床意义OACSTPCD
The expression and clinical significance of TIMP-3 and FN1 in nasopharyngeal carcinoma tissue
目的 探讨鼻咽癌患者基质金属蛋白酶抑制因子3(tissue inhibitor of matrix metalloproteinase-3,TIMP-3)、纤维连接蛋白1(fibronectin 1,FN1)的表达及临床意义.方法 选取2018年5月～2019年5月十堰市太和医院收治的102例鼻咽癌患者作为观察对象(病例组),另取102例慢性鼻咽炎患者作为对照组.利用免疫组化法测定两组TIMP-3、FN1表达.对患者预后进行随访,利用Kaplan-Meier法分析鼻咽癌组织TIMP-3、FN1表达水平与患者5年预后生存关系.影响因素利用Cox风险回归法分析.结果 病例组TIMP-3蛋白阳性率(25.49%,26/102)低于对照组(82.35%,84/102),差异有统计学意义(χ2=66.369,P<0.05);FN1蛋白阳性率(71.57%,73/102)高于对照组(20.59%,21/102),差异有统计学意义(χ2=53.348,P<0.05).TIMP-3、FN1表达与淋巴结转移、TNM分期有关(P<0.05).5年随访期间,102例鼻咽癌患者中64例无病生存为生存组,38例鼻咽癌患者发生死亡为死亡组.鼻咽癌组织TIMP-3阴性表达患者3年生存率(56.58%,43/76)低于TIMP-3阳性表达患者(80.77%,21/26)(χ2=8.921,P<0.05);鼻咽癌组织中FN1阴性表达患者3年生存率(79.31%,23/29)高于FN1阳性表达患者(56.16%,41/73)(χ2=8.596,P<0.05).FN1阳性、TNM分期及淋巴结转移是鼻咽癌患者死亡的独立危险因素,TIMP-3是鼻咽癌患者死亡的保护因素(P<0.05).结论 鼻咽癌患者组织中TIMP-3表达降低、FN1表达升高,两者与鼻咽癌进展与预后密切相关,或可作为鼻咽癌潜在的预后标志物.
OBJECTIVE To investigate the expression and clinical significance of tissue inhibitor of matrix metalloproteinase-3(TIMP-3)and fibronectin 1(FN1)in nasopharyngeal carcinoma patients.METHODS A total of 102 nasopharyngeal cancer patients admitted to Taihe Hospital from May 2018 to May 2019 were selected as the observation subjects(case group),and another 102 patients with chronic nasopharyngitis were selected as the control group.Immunohistochemistry was applied to determine the expression of TIMP-3 and FN1 in both groups.The prognosis of the patient is followed up.Kaplan-Meier method was applied to analyze the relationship between the expression levels of TIMP-3 and FN1 in nasopharyngeal carcinoma tissue with the 3-year prognostic survival of patients.The influencing factors were analyzed using Cox risk regression method.RESULTS The positive rate of TIMP-3 protein in the case group(25.49%,26/102)was lower than that in the control group(82.35%,84/102),with a statistically significant difference(χ2=66.369,P<0.05);the positive rate of FN1 protein(71.57%,73/102)was higher than that in the control group(20.59%,21/102),with a statistically significant difference(χ2=53.348,P<0.05).The expression of TIMP-3 and FN1 was related to lymph node metastasis and TNM staging(P<0.05).During the 5-year follow-up period,64 of 102 nasopharyngeal cancer patients with disease-free survival were categorized into the survival group,and 38 nasopharyngeal cancer patients who experienced death were categorized into the death group.The 5-year survival rate(56.58%,43/76)of patients with TIMP-3 negative expression in nasopharyngeal carcinoma tissue was lower than that of patients with TIMP-3 positive expression(80.77%,21/26)(χ2=8.921,P<0.05).The 5-year survival rate(23/29,79.31%)of patients with FN1 negative expression in nasopharyngeal carcinoma tissue was higher than that of patients with FN1 positive expression(41/73,56.16%)(χ2=8.596,P<0.05).FN1 positive,TNM staging,and lymph node metastasis were independent risk factors for mortality in nasopharyngeal carcinoma patients,while TIMP-3 was a protective factor for mortality in nasopharyngeal carcinoma patients(P<0.05).CONCLUSION TIMP-3 expression decreases and FN1 expression increases in tissues of nasopharyngeal carcinoma patients.Both are closely related to the progression and prognosis of nasopharyngeal carcinoma,and may serve as potential prognostic markers for nasopharyngeal carcinoma.
曹成;余滋中;许昱
十堰市太和医院耳鼻咽喉头颈外科,湖北 十堰 442000十堰市太和医院耳鼻咽喉头颈外科,湖北 十堰 442000湖北省人民医院耳鼻咽喉头颈外科,湖北 武汉 430060
鼻咽癌预后基质金属蛋白酶3纤维连接蛋白1
Nasopharyngeal CarcinomaPrognosistissue inhibitor of matrix metalloproteinase-3fibronectin 1
《中国耳鼻咽喉头颈外科》 2024 (11)
703-707,5
2022年十堰市引导科研项目(22Y37)
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