渔业研究2024，Vol.46Issue(6)：580-591,12.DOI:10.14012/j.jfr.2024122
双斑东方鲀鱼皮胶原多肽对UVB辐射小鼠成纤维细胞的光损伤修复功效
Photoprotective effect of collagen peptides from the skin of Takifugu bimaculatus on UVB-induced damage in mouse fibroblasts
摘要
Abstract
[Background]Takifugu bimaculatus is one of the main species of cultured puffer fish in Fujian Province.The significant increase in its production in recent years has led to an increasingly urgent need for high-value utilization of processing by-products such as its skin.[Objective]Long-term exposure to ultraviolet B(UVB)radiation leads to skin inflammation and a reduction in dermal collagen,among other photodamage phenomena.This study utilized UVB-irradiated mouse fibroblasts as a model for photodamage to investigate the reparative effects of low molecular weight collagen peptides derived from T.bimaculatus fish skin(TBCP-L)on UVB-exposed L929 cells.[Methods]The study first verified the safety of TBCP through in vitro models.Sub-sequently,TBCP-L with a molecular weight less than 3 kD was obtained through ultrafiltration.Cell viability assays were conducted to evaluate the preventive and reparative effects of TBCP-L on UVB-irradiated L929 cells.Real-time quantitative polymerase chain reaction(qPCR)was used to detect the messenger ribonucleic acid(mRNA)expression levels of inflammatory cytokines and matrix metalloproteinases(MMPs)in UVB-ex-posed L929 cells.The wound healing effect of TBCP-L was studied through a cell scratch assay.[Results]Epi-skinTM 3D models and bovine corneal experiments indicated that 1.5 g/mL TBCP solution was non-irritating to skin and eyes.The molecular weight distribution results indicated that the weight-average molecular weight of TBCP-L was 2 352 Da,with 80.2%of the components having a molecular weight below 2 200 Da.A 2 mg/mL TBCP-L concentration was non-toxic to L929 cells and did not prevent UVB radiation damage but exhibited significant reparative activity.qPCR results indicated that TBCP-L could significantly inhibit the upregulated expression of inflammatory cytokines such as interleukin-1β,interleukin-6,tumor necrosis factor-α and cyc-looxygenase-2 and MMP2,MMP3,and MMP9 in L929 cells following UVB stimulation.Additionally,incubation with 500 μg/mL TBCP-L for 96 h significantly accelerated the scratch repair rate of L929 cells,with a migration rate reaching 37.8%±3.3%.[Conclusion]In conclusion,TBCP-L may accelerate the repair of UVB-irradiated L929 cells by inhibiting inflammatory responses and collagen loss,and it is non-irritating to the skin and eyes.It is a potential candidate for anti-aging and post-sun exposure repair cosmetic ingredients.[Significance]This study aims to investigate the potential of TBCP-L as a cosmetic ingredient for mitigating photoaging and post-sun exposure repair,providing robust theoretical support for its efficacy.Additionally,it holds significant prac-tical implications for the high-value utilization of puffer fish resources and the sustainable development of the industry.关键词
双斑东方鲀/胶原多肽/紫外线/光保护Key words
Takifugu bimaculatus/collagen peptide/ultraviolet/photoprotective分类
农业科技引用本文复制引用
陈贝,薛凯琳,汤海燕,张鸽,乔琨,许旻,张东,刘智禹..双斑东方鲀鱼皮胶原多肽对UVB辐射小鼠成纤维细胞的光损伤修复功效[J].渔业研究,2024,46(6):580-591,12.基金项目
福建省自然科学基金项目(2023J011324) （2023J011324）
厦门市自然科学基金项目(3502Z20227304) （3502Z20227304）
厦门市海洋与渔业发展专项资金青年科技创新项目(23YYZP028QCB27) （23YYZP028QCB27）
福建省促进海洋与渔业高质量发展专项资金项目(FJHYF-L-2023-22、FJHYF-L-2023-24) （FJHYF-L-2023-22、FJHYF-L-2023-24）
