渔业研究2024，Vol.46Issue(6)：626-634,9.DOI:10.14012/j.jfr.2024056
温度波动对大刺鳅抗氧化指标和热休克蛋白基因表达的影响
Effects of temperature fluctuations on antioxidant indices and heat shock protein gene expressions in Mastacembelus armatus
摘要
Abstract
[Objective]Ambient temperature changes,particularly cooling,can induce metabolic disorders in fish,resulting in a substantial accumulation of free radicals.This,in turn,affects the normal physiological func-tions and immune defense mechanisms of fish cells and tissues.This study aimed to investigate the stress response of fish to abrupt temperature changes.[Methods]Mastacembelus armatus was used as the experimental model.The study simulated the stress response of fish under temperature fluctuations by implementing a temperature change protocol(initial temperature group:28 ℃→low-temperature maintenance group:20 ℃→recovery tem-perature group:28 ℃).The effects of these temperature fluctuations on antioxidant indices and heat shock pro-tein gene expression were assessed.[Results]The activities of superoxide dismutase(SOD)and catalase(CAT)in the liver of M.armatus significantly decreased during temperature fluctuations(P<0.05).Total antiox-idant capacity(T-AOC)increased in the low-temperature maintenance group,but no significant difference were observed among the three groups(P>0.05).Malondialdehyde(MDA)levels significantly increased in the low-temperature maintenance group(P<0.05),but returned to initial levels in the recovery temperature group.Com-pared to the initial temperature group,the expression level of hsp60 in gill tissue was significantly reduced in the low-temperature maintenance group and increased significantly in the recovery temperature group(P<0.05).The expression of hsc70 in gill tissue showed a significant downward trend in the low-temperature maintenance group(P<0.05);however,in brain tissue,the expression of hsc70 in the recovery temperature group was signi-ficantly lower compared to the low-temperature maintenance group(P<0.05).The expression of hsp70 in gills and brain tissue significantly decreased in the low-temperature maintenance group and significantly increased in the recovery temperature group(P<0.05).Conversely,the expression of hsp90 in both gills and brain tissues significantly increased in both the low-temperature maintenance and recovery temperature groups(P<0.05).[Conclusion]This study demonstrates that rapid temperature fluctuations in aquatic environments can induce oxidative stress responses and affect the expression of heat shock protein genes in different tissues of M.armatus.[Significance]The findings provide valuable data and theoretical insights for ecological domestication,healthy breeding practices,environmental management,and value-added maintenance of M.armatus.关键词
大刺鳅/温度波动/抗氧化酶/酶活力/热休克蛋白基因Key words
Mastacembelus armatus/temperature fluctuation/antioxidant enzymes/enzyme activity/heat shock protein genes分类
农业科技引用本文复制引用
梁海燕,束庆松,王充,关歆,丁春华,邹记兴,周爱国..温度波动对大刺鳅抗氧化指标和热休克蛋白基因表达的影响[J].渔业研究,2024,46(6):626-634,9.基金项目
广州市增城区农业农村局增江大刺鳅种质资源调查项目(HT2022113) （HT2022113）
广东省中央财政农业技术推广项目(F16271) （F16271）
华南农业大学青年科技人才培育专项基金(201707N025) （201707N025）
华南农业大学引进人才专项经费(K16226) （K16226）
