温度波动对大刺鳅抗氧化指标和热休克蛋白基因表达的影响OA

[Objective]Ambient temperature changes,particularly cooling,can induce metabolic disorders in fish,resulting in a substantial accumulation of free radicals.This,in turn,affects the normal physiological func-tions and immune defense mechanisms of fish cells and tissues.This study aimed to investigate the stress response of fish to abrupt temperature changes.[Methods]Mastacembelus armatus was used as the experimental model.The study simulated the stress response of fish under temperature fluctuations by implementing a temperature change protocol(initial temperature group:28 ℃→low-temperature maintenance group:20 ℃→recovery tem-perature group:28 ℃).The effects of these temperature fluctuations on antioxidant indices and heat shock pro-tein gene expression were assessed.[Results]The activities of superoxide dismutase(SOD)and catalase(CAT)in the liver of M.armatus significantly decreased during temperature fluctuations(P<0.05).Total antiox-idant capacity(T-AOC)increased in the low-temperature maintenance group,but no significant difference were observed among the three groups(P>0.05).Malondialdehyde(MDA)levels significantly increased in the low-temperature maintenance group(P<0.05),but returned to initial levels in the recovery temperature group.Com-pared to the initial temperature group,the expression level of hsp60 in gill tissue was significantly reduced in the low-temperature maintenance group and increased significantly in the recovery temperature group(P<0.05).The expression of hsc70 in gill tissue showed a significant downward trend in the low-temperature maintenance group(P<0.05);however,in brain tissue,the expression of hsc70 in the recovery temperature group was signi-ficantly lower compared to the low-temperature maintenance group(P<0.05).The expression of hsp70 in gills and brain tissue significantly decreased in the low-temperature maintenance group and significantly increased in the recovery temperature group(P<0.05).Conversely,the expression of hsp90 in both gills and brain tissues significantly increased in both the low-temperature maintenance and recovery temperature groups(P<0.05).[Conclusion]This study demonstrates that rapid temperature fluctuations in aquatic environments can induce oxidative stress responses and affect the expression of heat shock protein genes in different tissues of M.armatus.[Significance]The findings provide valuable data and theoretical insights for ecological domestication,healthy breeding practices,environmental management,and value-added maintenance of M.armatus.