海南医学院学报2024，Vol.30Issue(23)：1776-1783,8.DOI:10.13210/j.cnki.jhmu.20240829.001
基于METTL3调控STAT1的m6A甲基化探讨梅花生津饮抑制巨噬细胞M1型极化的分子机制
Molecular mechanism of Meihua Shengjin decoction in inhibiting M1 macrophage polarization via METTL3-regulated m6A methylation of STAT1
摘要
Abstract
Objective:To explore the effect of Meihua Shengjin decoction on METTL3-mediated m6A methylation of STAT1,and to elucidate its molecular mechanism in treating primary sjögren's syndrome by modulating macrophage polarization.Methods:An in vitro inflammation model was established using LPS-induced RAW264.7,and Meihua Shengjin decoction-containing serum was administered for intervention.Flow cytometry was used to assess the proportion of M1 macro-phages,ELISA to measure the levels of inflammatory factors(IL-6,IL-23,TNF-α)in cell supernatants,Western blot and RT-qPCR to detect the expression of STAT1,METTL3,and glycolysis-related proteins and genes,and dot blotting to evaluate the level of m6A methylation level of STAT1.Results:Compared with the control group,LPS induction significantly increased the proportion of M1 macrophages and the levels of IL-6,IL-23,and TNF-α in the cell culture supernatant,and upregulated the mRNA and protein expression of METTL3,STAT1,GP1,and ALDOA,with statistically significant differences(P<0.01).Compared with the model group,Meihua Shengjin decoction-containing serum significantly reduced the proportion of M1 macro-phages and the levels of IL-6,IL-23,and TNF-α in the cell culture supernatant,and downregulated the mRNA and protein ex-pression of METTL3,STAT1,GP1,and ALDOA,with statistically significant differences(P<0.05 and P<0.01).Additional-ly,LPS induction significantly increased the m6A methylation modification level of STAT1 compared to the control group(P<0.01),while Meihua Shengjin decoction-containing serum significantly reduced the m6A methylation level in macrophages,with statistically significant differences compared to the model group(P<0.01).Conclusion:Meihua Shengjin decoction inhibits MET-TL3 expression and reduces STAT1 m6A methylation,thereby suppressing M1 macrophage polarization and inflammation by low-ering glycolysis levels in macrophages.关键词
巨噬细胞极化/m6A甲基化/梅花生津饮/METTL3/干燥综合征Key words
Macrophage polarization/m6A methylation/Meihua Shengjin decoction/METTL3/Primary Sjögren's syn-drome分类
医药卫生引用本文复制引用
夏凯雨,李奇玮,李泽光,李添,刘茜茜,张思宇,梁华..基于METTL3调控STAT1的m6A甲基化探讨梅花生津饮抑制巨噬细胞M1型极化的分子机制[J].海南医学院学报,2024,30(23):1776-1783,8.基金项目
This study was supported by Academic Inheritance and Research Project of Master of Traditional Chinese Medicine(GY2022-02) 国医大师学术传承科研课题(GY2022-02) （GY2022-02）
