海南医学院学报2024，Vol.30Issue(23)：1792-1797,6.DOI:10.13210/j.cnki.jhmu.20240708.001
GPX4基因多态性与幽门螺杆菌感染的关联研究
Association analysis of GPX4 gene polymorphisms with Helicobacter pylori infection
摘要
Abstract
Objective:To analyze the association of single nucleotide polymorphisms(SNP)in glutathione peroxidase 4(GPX4)gene with Helicobacter pylori(H.pylori)infection.Methods:A total of 506 samples were selected to detect H.pylori in-fection by enzyme-linked immunosorbent assay(ELISA)and 14C Urea breath test(UBT),a positive result of either test was deter-mined as positive;GPX4 rs4807542,rs713041,rs713041 and rs3746162 were genotyped by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method;Unconditional logistic regression was used to analyze the association of each SNP with H.pylori infection.SHEsis software was used to constructed Haplotype to analyze its association with H.pylori in-fection.The relationship between SNP-SNP interaction and H.pylori infection was analyzed by generalized multi-factor dimen-sionality reduction method.Results:GPX4 rs4807542,rs713041,rs2074451 and rs3746162 were not associated with H.pylori in-fection in codominant,dominant,recessive,and overdominant models(P>0.05);Haplotypes constructed by rs4807542,rs713041,rs2074451 and rs3746162 were not founded to be associated with H.pylori infection,and these SNPs also did not have interaction in H.pylori infection(P>0.05).Conclusion:GPX4 rs4807542,rs713041,rs2074451 and rs3746162 may not play a major role in the process of H.pylori infection in Baotou Han population.关键词
谷胱甘肽过氧化酶4/单核苷酸多态性/幽门螺杆菌/单体型Key words
Glutathione peroxidase 4/Single nucleotide polymorphisms/Helicobacter pylori/Haplotypes分类
医药卫生引用本文复制引用
陈雅茹,高芳,董文杰,褚宁,贾彦彬..GPX4基因多态性与幽门螺杆菌感染的关联研究[J].海南医学院学报,2024,30(23):1792-1797,6.基金项目
This study was supported by the Nature Fund of Inner Mongolia(No.2024MS08079、No.2021MS08054) （No.2024MS08079、No.2021MS08054）
Innovation team of Baotou Medical College(No.bycxtd-06)and Scientific Research Fund Project of Baotou Medical College(No.BYJJ-GDJY202313) 内蒙古自然科学基金资助项目(2024MS08079、2021MS08054) （No.bycxtd-06）
包头医学院创新团队(bycxtd-06) （bycxtd-06）
包头医学院科学研究基金项目(BYJJ-GDJY202313) （BYJJ-GDJY202313）
