河南农业大学学报2024，Vol.58Issue(6)：919-927,9.DOI:10.16445/j.cnki.1000-2340.20240418.002
玉米抗穗腐病基因ZmRpp13-34的鉴定及功能分析
Identification and functional analysis of ear rot resistance gene ZmRpp13-34 in maize
摘要
Abstract
[Objective]This study aims to identify the members of maize Rpp13(Recognition of Peronospora parasitica 13)gene family associated with resistance to maize ear rot resistance,thereby laying a foundation for enriching maize ear rot resistance gene resources.[Method]Through association analysis,transcriptome analysis,real-time fluorescence quantitative PCR(RT-qPCR),candidate gene analysis and haplotype analysis,the identification and functional analysis of maize ear rot resistance genes were carried out.[Result]Fifty-six maize Rpp13 genes were identified from the maize genome and named as ZmRpp13-1 through ZmRpp13-56 according to their physical order.Through candidate gene association analysis between natural variation sites in maize Rpp13 genes and resistance to ear rot disease,several significant candidate genes conferring resistance to ear rot were discovered within the maize Rpp13 gene family.Zm00001d037650(ZmRpp13-34)showed the most pronounced association with resistance to maize ear rot based on RNA sequencing and analysis.RT-qPCR revealed that the relative expression level of ZmRpp13-34 was significantly upregulated post-inoculation with Fusarium verticillioides.The Haplotype analysis of ZmRpp13-34 pinpointed functional haplotypes affecting resistance to ear rot.[Conclusion]The results showed that the genetic variation of maize Rpp13 gene was significantly correlated with ear rot resistance,and ZmRpp13-34 emerges as a critical candidate gene involved in regulating maize resistance to ear rot.关键词
玉米/穗腐病/抗性/Rpp13基因家族/关联分析Key words
maize/ear rot/resistance/Rpp13 gene family/association analysis分类
农业科技引用本文复制引用
王慧娟,宋云霞,滕慧鑫,董朝沛,吴亚滨,高景阳,周子键,袁祖丽,吴建宇..玉米抗穗腐病基因ZmRpp13-34的鉴定及功能分析[J].河南农业大学学报,2024,58(6):919-927,9.基金项目
国家自然科学基金项目(32172034) （32172034）
