玉米抗穗腐病基因ZmRpp13-34的鉴定及功能分析OA北大核心CSTPCD

[Objective]This study aims to identify the members of maize Rpp13(Recognition of Peronospora parasitica 13)gene family associated with resistance to maize ear rot resistance,thereby laying a foundation for enriching maize ear rot resistance gene resources.[Method]Through association analysis,transcriptome analysis,real-time fluorescence quantitative PCR(RT-qPCR),candidate gene analysis and haplotype analysis,the identification and functional analysis of maize ear rot resistance genes were carried out.[Result]Fifty-six maize Rpp13 genes were identified from the maize genome and named as ZmRpp13-1 through ZmRpp13-56 according to their physical order.Through candidate gene association analysis between natural variation sites in maize Rpp13 genes and resistance to ear rot disease,several significant candidate genes conferring resistance to ear rot were discovered within the maize Rpp13 gene family.Zm00001d037650(ZmRpp13-34)showed the most pronounced association with resistance to maize ear rot based on RNA sequencing and analysis.RT-qPCR revealed that the relative expression level of ZmRpp13-34 was significantly upregulated post-inoculation with Fusarium verticillioides.The Haplotype analysis of ZmRpp13-34 pinpointed functional haplotypes affecting resistance to ear rot.[Conclusion]The results showed that the genetic variation of maize Rpp13 gene was significantly correlated with ear rot resistance,and ZmRpp13-34 emerges as a critical candidate gene involved in regulating maize resistance to ear rot.