河南农业大学学报2024，Vol.58Issue(6)：936-945,10.DOI:10.16445/j.cnki.1000-2340.20240429.007
枣DCL基因家族注释及其在枣疯病发生中的表达模式分析
Annotation of jujube DCL gene family and its expression pattern during jujube witches'broom disease
摘要
Abstract
[Objective]The members of the jujube DCL gene family were annotated from the jujube ge-nome,and their expression patterns in the occurrence of jujube witches'broom disease were analyzed,and the role of the jujube DCL gene family in responding to phytoplasma infection was studied.[Method]The genome data of Jujube(Genebank No.JREP00000000)was downloaded from the NCBI website,and the DCL family members of Jujube were screened by bioinformatics methods using the DCL sequence of Arabidopsis thaliana as probe,and their chromosomal position,gene and protein struc-ture,phylogenetic and conserved motif characteristics were analyzed,the transcriptome data of the DCL gene family members of jujube during the progress of jujube witches'broom disease were analyzed,and the dynamics of each member in the process of jujube witches'broom disease were quantified by qRT-PCR.[Result]Eleven jujube DCLs(ZjDCLs)were annotated using four Arabidopsis DCL proteins as probes,with molecular weights ranging from 15217.48～225623.49 Da and isoelectric points ranging from 5.82～9.05.The 11 jujube DCL genes were unevenly distributed on 6 jujube chromosomes,most of which were on chromosome 1,and the 11 jujube DCLs could be divided into 4 categories according to the evolutionary relationship with the 4 DCLs in Arabidopsis,and the gene structure analysis showed that the number of ZjDCL3 and ZjDCL7 exons was up to 25,and the conserved motif analysis showed that the structure of jujube DCL was relatively conserved.Transcriptome data analysis showed that 10 jujube DCL genes were expressed during the process of jujube witches'broom disease,and the expre-ssion level of ZjDCL6 was the highest in the Ⅳ period,and qRT-PCR quantitative analysis showed that ZjDCL6,ZjDCL8 and ZjDCL10 were significantly different between healthy and diseased trees at diffe-rent periods.[Conclusion]There were 11 DCL gene family members in the jujube genome,and the structure was relatively conserved,among which ZjDCL6,ZjDCL8 and ZjDCL10 played an important role in the response of jujube to phytoplasma infection.关键词
枣疯病/植原体/DCL基因家族/植物防御/表达模式Key words
jujube witches'broom disease/phytoplasma/DCL gene family/plant defense/expression pattern分类
农业科技引用本文复制引用
张亚婕,黄玉静,姚佳,曹懂懂,李君毅,王梦丽,李萌,李继东,冯建灿..枣DCL基因家族注释及其在枣疯病发生中的表达模式分析[J].河南农业大学学报,2024,58(6):936-945,10.基金项目
国家自然科学基金项目(32071803) （32071803）
河南省自然科学基金项目(232300420007) （232300420007）
河南省科技兴林项目(2023008) （2023008）
