河南农业大学学报2024，Vol.58Issue(6)：946-956,11.DOI:10.16445/j.cnki.1000-2340.20240407.002
药百合LsSPX1基因的克隆及表达分析
Cloning and expression analysis of LsSPX1 gene in Lilium speciosum var.gloriosoides
摘要
Abstract
[Objective]To clone and analyze the expression pattern of SPX gene of Lilium speciosum var.gloriosoides,and to explore its potential regulatory function on the formation of color of Lilium spe-ciosum var.gloriosoides.[Methods]In this study,LsSPX1 gene was cloned from Lilium speciosum var.gloriosoides using RT-PCR.Bioinformatics,subcellular localization and quantitative real-time PCR(qRT-PCR)analyses were performed to explore the functional characteristics of LsSPX1 gene in lily flower color formation.[Results]The coding region of LsSPX1 gene was 822 bp in length,enco-ding 273 amino acids.LsSPX1 protein contains an N-terminal SPX domain.Phylogenetic analysis showed that LsSPX1 belonged to the SPX subfamily with one SPX domain,and was most closely relat-ed to the SPX1 of Dendrobium officinale and Phalaenopsis equestris.Subcellular localization showed that LsSPX1 was mainly located in the nucleus.qRT-PCR fluorescence quantitative analysis showed that LsSPX1 was up-regulated during the development of the colorless regions of the tepals,and re-mained at relatively low levels in the colored regions at the same stage.A potential gene regulatory net-work constructed using GENIE3 indicated that LsSPX1 is co-expressed with multiple transcription fac-tors that negatively regulate anthocyanin synthesis.[Conclusion]LsSPX1 may have a negative regula-tory function on anthocyanin biosynthesis and play an important role in the formation of the colorless re-gions in the tepals of Lilium speciosum var.gloriosoides.关键词
药百合/SPX基因/花青素/克隆/基因表达Key words
Lilium speciosum var.gloriosoides/SPX gene/anthocyanins/cloning/gene expression分类
农业科技引用本文复制引用
孙婷婷,刘筱琳,杨捷,赵一燃,朱云涛,何恒斌..药百合LsSPX1基因的克隆及表达分析[J].河南农业大学学报,2024,58(6):946-956,11.基金项目
国家重点研发计划(2019YFD1000400) （2019YFD1000400）
国家自然科学基金项目(31672191) （31672191）
