首页|期刊导航|河南农业大学学报|白藜芦醇对鼠伤寒沙门氏菌诱导型IPEC-J2细胞损伤的缓解作用

白藜芦醇对鼠伤寒沙门氏菌诱导型IPEC-J2细胞损伤的缓解作用

赵峥余秋颖杨聪燕李珊珊陈琳琳王娜

河南农业大学学报2024，Vol.58Issue(6)：957-965,9.

河南农业大学学报2024，Vol.58Issue(6)：957-965,9.DOI:10.16445/j.cnki.1000-2340.20240328.001

白藜芦醇对鼠伤寒沙门氏菌诱导型IPEC-J2细胞损伤的缓解作用

Alleviating effect of resveratrol on Salmonella typhimurium-induced damage in IPEC-J2 cell

赵峥 ¹余秋颖 ²杨聪燕 ³李珊珊 ³陈琳琳 ²王娜⁴

作者信息

1. 河南农业大学食品科学技术学院,河南郑州 450002||郑州市营养与健康食品重点实验室,河南郑州 450002
2. 河南农业大学食品科学技术学院,河南郑州 450002||郑州市营养与健康食品重点实验室,河南郑州 450002||龙湖现代免疫实验室,河南郑州 450046||河南农业大学动物免疫学国际联合研究中心,河南郑州 450046
3. 龙湖现代免疫实验室,河南郑州 450046||河南农业大学动物免疫学国际联合研究中心,河南郑州 450046||河南农业大学动物医学院,河南郑州 450046
4. 郑州市营养与健康食品重点实验室,河南郑州 450002||龙湖现代免疫实验室,河南郑州 450046||河南农业大学动物免疫学国际联合研究中心,河南郑州 450046||河南农业大学动物医学院,河南郑州 450046
折叠

摘要

Abstract

[Objective]To investigate the protective effects of resveratrol(RES)against oxidative and inflammatory damage induced by Salmonella typhimurium in intestinal porcine epithelial(IPEC-J2)cells and provide insights to enhance animal health in breeding practices.[Method]An IPEC-J2 cell injury model was established using S.typhimurium,and key oxidative stress and inflammatory indicators were measured in the cell supernatant,including nitric oxide(NO)content,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-4(IL-4)secretion,lactate dehydrogenase(LDH)level,total superoxide dismutase(T-SOD),reduced glutathione(GSH)and malondialdehyde(MDA),to analyze the mitigating effects of RES on IPEC-J2 cell injury model.[Result]Pretreatment of IPEC-J2 cells with RES at an optimal mass concentration of 7.8 mg·L-1 could significantly alleviate the inflammatory injury induced by S.typhimurium,notably reducing the contents of pro-inflammatory factors(TNF-α and IL-6),and increasing the expression of inhibitory factors(IL-4),thus effectively slowing down the intestinal inflammatory response.Meanwhile,RES could significantly increase the activities of T-SOD and GSH enzymes,significantly reduce the expression level of MDA,and alleviate the oxidative damage induced by S.typhimurium,thereby contributing to the maintenance of intestinal redox homeostasis of the organism.[Conclusion]RES has a protective effect on IPEC-J2 cell damage and holds potential for preventing S.typhimurium infection in animal models.

关键词

白藜芦醇/鼠伤寒沙门氏菌/IPEC-J2细胞/炎症反应/氧化应激

Key words

resveratrol/Salmonella typhimurium/IPEC-J2 cells/inflammatory response/oxidative stress

分类

轻工纺织

引用本文复制引用

赵峥,余秋颖,杨聪燕,李珊珊,陈琳琳,王娜..白藜芦醇对鼠伤寒沙门氏菌诱导型IPEC-J2细胞损伤的缓解作用[J].河南农业大学学报,2024,58(6):957-965,9.

基金项目

河南省重大科技专项(221100110600) （221100110600）

河南省创新生态支撑专项(HARS-22-05-Z1) （HARS-22-05-Z1）

河南农业大学学报

OA北大核心CSTPCD

ISSN：1000-2340

访问量0

下载量0

段落导航