白藜芦醇对鼠伤寒沙门氏菌诱导型IPEC-J2细胞损伤的缓解作用OA北大核心CSTPCD

[Objective]To investigate the protective effects of resveratrol(RES)against oxidative and inflammatory damage induced by Salmonella typhimurium in intestinal porcine epithelial(IPEC-J2)cells and provide insights to enhance animal health in breeding practices.[Method]An IPEC-J2 cell injury model was established using S.typhimurium,and key oxidative stress and inflammatory indicators were measured in the cell supernatant,including nitric oxide(NO)content,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-4(IL-4)secretion,lactate dehydrogenase(LDH)level,total superoxide dismutase(T-SOD),reduced glutathione(GSH)and malondialdehyde(MDA),to analyze the mitigating effects of RES on IPEC-J2 cell injury model.[Result]Pretreatment of IPEC-J2 cells with RES at an optimal mass concentration of 7.8 mg·L-1 could significantly alleviate the inflammatory injury induced by S.typhimurium,notably reducing the contents of pro-inflammatory factors(TNF-α and IL-6),and increasing the expression of inhibitory factors(IL-4),thus effectively slowing down the intestinal inflammatory response.Meanwhile,RES could significantly increase the activities of T-SOD and GSH enzymes,significantly reduce the expression level of MDA,and alleviate the oxidative damage induced by S.typhimurium,thereby contributing to the maintenance of intestinal redox homeostasis of the organism.[Conclusion]RES has a protective effect on IPEC-J2 cell damage and holds potential for preventing S.typhimurium infection in animal models.