河南农业大学学报2024，Vol.58Issue(6)：966-975,10.DOI:10.16445/j.cnki.1000-2340.20240511.001
蒸汽爆破忍冬藤饲料添加剂的益生功效研究
Study on the probiotic effect of steam exploded Lonicerae japonicae Caulis feed additive
摘要
Abstract
[Objective]Exploring the probiotic effect of steam exploded Lonicerae japonicae Caulis(LJC),and providing theoretical basis for the application of steam exploded LJC feed additives.[Method]Taking four steam exploded treatments(SELC-HOX,SELC-HCA,SELC-M1,and SELC-M2)of LJC as the research subjects,the study utilized the 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)methods to comparatively ana-lyze the free radical scavenging capacity of different steam exploded LJC.Additionally,the impact of different steam exploded LJC on the diversity and composition of pig intestinal microbiota was investi-gated through in vitro simulated experiments and 16S rRNA gene amplicon sequencing technology.[Result]Compared to untreated LJC,the steam exploded counterpart exhibited stronger free radical scavenging ability.The results of in vitro simulation experiments showed that steam explored LJC could significantly reduce the relative abundance of Clostridium_sensu_stricto_1,pathogenic Esche-richia-Shigella and Citrobacter,and significantly increased the relative abundance of lactobacillus in the pig intestine.The free radical scavenging ability of steam exploded LJC,as well as the relative abundance of probiotic genera such as Lactobacillus,Streptococcus,Eenterococcus and Bifidobacterium in the pig intestine,positively correlated with xylo-oligosaccharide content of LJC.While the relative abundance of Clostridium_sensu_stricto_1,pathogenic Escherichia-Shigella and Citrobacter negatively correlated with xylo-oligosaccharide content of LJC.[Conclusion]Steam exploded LJC(SELC-HOX),exhibits stronger free radical scavenging ability and modulation effects on gut microbiota com-pared to unexploded ones,the probiotic effect significant,suggesting its potential application as a feed additive in animal husbandry.关键词
忍冬藤/蒸汽爆破/低聚木糖/绿原酸/益生功效Key words
Lonicerae japonicae Caulis/steam explosion/xylo-oligosaccharides/chlorogenic acid/probiotic effect分类
农业科技引用本文复制引用
孙榕泽,张旭屹,彭仕乐,谢慧,毛国涛,宋安东,张宏森,王风芹..蒸汽爆破忍冬藤饲料添加剂的益生功效研究[J].河南农业大学学报,2024,58(6):966-975,10.基金项目
河南省科技攻关项目(222102520015) （222102520015）
中原科技创新领军人才项目(204200510018) （204200510018）
