蒸汽爆破忍冬藤饲料添加剂的益生功效研究OA北大核心CSTPCD

[Objective]Exploring the probiotic effect of steam exploded Lonicerae japonicae Caulis(LJC),and providing theoretical basis for the application of steam exploded LJC feed additives.[Method]Taking four steam exploded treatments(SELC-HOX,SELC-HCA,SELC-M1,and SELC-M2)of LJC as the research subjects,the study utilized the 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)methods to comparatively ana-lyze the free radical scavenging capacity of different steam exploded LJC.Additionally,the impact of different steam exploded LJC on the diversity and composition of pig intestinal microbiota was investi-gated through in vitro simulated experiments and 16S rRNA gene amplicon sequencing technology.[Result]Compared to untreated LJC,the steam exploded counterpart exhibited stronger free radical scavenging ability.The results of in vitro simulation experiments showed that steam explored LJC could significantly reduce the relative abundance of Clostridium_sensu_stricto_1,pathogenic Esche-richia-Shigella and Citrobacter,and significantly increased the relative abundance of lactobacillus in the pig intestine.The free radical scavenging ability of steam exploded LJC,as well as the relative abundance of probiotic genera such as Lactobacillus,Streptococcus,Eenterococcus and Bifidobacterium in the pig intestine,positively correlated with xylo-oligosaccharide content of LJC.While the relative abundance of Clostridium_sensu_stricto_1,pathogenic Escherichia-Shigella and Citrobacter negatively correlated with xylo-oligosaccharide content of LJC.[Conclusion]Steam exploded LJC(SELC-HOX),exhibits stronger free radical scavenging ability and modulation effects on gut microbiota com-pared to unexploded ones,the probiotic effect significant,suggesting its potential application as a feed additive in animal husbandry.