海洋科学2024，Vol.48Issue(10)：1-12,12.DOI:10.11759/hykx20230410001
浒苔交替氧化酶(alternative oxidase)基因克隆与环境应答
Cloning and environmental response of the alternative oxi-dase gene in Ulva prolifera
摘要
Abstract
As the dominant species of the green tide in the Yellow Sea,Ulva prolifera has strong adaptability to adversity.Alternative oxidase(AOX)plays an important role during plant stress response.To explore the structure,function,and environmental response characteristics of AOX in U.prolifera,the rapid amplification of cDNA ends(RACE)technique was used to characterize its full-length AOX(UpAOX)and bioinformatics to analyze its sequence.Simultaneously,the differences in the expression of AOX in U.prolifera under different environments were ex-plored.The full-length cDNA of AOX was 2,441 bp,which encoded 236 amino acids.AOX protein had a typical"EXXH"and"EEE-Y"iron ion binding conserved motif.AOX was highly conserved during evolution,especially the C-terminal.AOX contained cis-acting elements related to environmental stress response,growth and develop-ment,and signal transduction.The real-time fluorescence quantitative PCR results indicated that temperature,des-iccation,salinity,and UVB stresses were significantly correlated to AOX transcription.These results provided a theoretical basis for further exploring the biological function and stress regulation mechanism of AOX in U.prolif-era.关键词
浒苔/交替氧化酶/基因克隆/生物信息学分析/转录分析/非生物胁迫Key words
Ulva prolifera/alternative oxidase/gene cloning/bioinformatics analysis/transcription analysis/abiotic stress分类
农业科技引用本文复制引用
许金慧,赵新宇,曲同飞,孙白雪,唐学玺,王影..浒苔交替氧化酶(alternative oxidase)基因克隆与环境应答[J].海洋科学,2024,48(10):1-12,12.基金项目
国家重点R&D项目(2022YFC3106001) （2022YFC3106001）
国家自然科学基金(41906120 （）
42176204 （）
41976132 （）
41706121) （）
国家自然科学基金-山东联合基金(U1806213 （）
U160640) （）
中央高校基础研究基金(201964025)[National Key R&D Program of China,No.2022YFC3106001 （201964025）
National Natural Science Foundation of China,Nos.41906120,42176204,41976132,41706121 （）
NSFC-Shandong Joint Fund,Nos.U1806213,U1606404 （）
Fundamental Research Funds for the Central Universities,No.201964025)] （）
