实用临床医药杂志2024，Vol.28Issue(22)：35-40,6.DOI:10.7619/jcmp.20242184
长链非编码RNA ZEB1反义RNA1通过靶向微小RNA-224调控淋巴瘤细胞增殖、侵袭、迁移的分子机制研究
Molecular mechanisms of long non-coding RNA ZEB1-antisense RNA1 in regulating lymphoma cell proliferation,invasion,and migration by targeting microRNA-224
摘要
Abstract
Objective To investigate the molecular mechanisms of long non-coding RNA ZEB1-antisense RNA1(LncRNA ZEB1-AS1)in regulating lymphoma cell proliferation,invasion,and mi-gration by targeting microRNA(miR)-224.Methods The lymphoma cell line Raji was cultured in vitro and transfected with si-NC,si-ZEB1-AS1,miR-NC,miR-224 mimics,si-ZEB1-AS1+anti-miR-NC,and si-ZEB1-AS1+anti-miR-224,respectively.Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR)was used to assess the expression levels of LncRNA ZEB1-AS1 and miR-224 in lymphoma cells.Cell viability was detected using the Cell Counting Kit-8(CCK-8).Cell migra-tion and invasion abilities were evaluated by Transwell experiments.Dual luciferase reporter experiments were conducted to confirm the regulatory effect of LncRNA ZEB1-AS1 on miR-224.Western blot was performed to detect the expression levels of cyclin D1(CyclinD1),matrix metalloproteinase(MMP)-2,and MMP-9 proteins.Results Compared with the control group,the lymphoma group showed increased LncRNA ZEB1-AS1 levels and decreased miR-224 expression levels(P＜0.05).Compared with the si-NC group,the si-ZEB1-AS1 group exhibited decreased expression levels of LncRNA ZEB1-AS1,optical density(OD)values,the number of migrated and invaded cells,and expression levels of CyclinD1,MMP-2,and MMP-9 protein(P＜0.05).Compared with the miR-NC group,the miR-224 group showed increased miR-224 expression levels but decreased OD val-ues,the number of migrated and invaded cells,and expression levels of CyclinD1,MMP-2,and MMP-9 proteins(P＜0.05).Upregulation of miR-224 levels reduced the luciferase activity of WT-ZEB1-AS1 but had no effect on MUT-ZEB1-AS1 luciferase activity.Compared with the pcDNA-NC group,the pcDNA-ZEB1-AS1 group showed decreased miR-224 expression levels(P＜0.05);compared with the si-NC group,the si-ZEB1-AS1 group showed increased miR-224 expression levels(P＜0.05).Compared with the si-ZEB1-AS1+anti-miR-NC group,the si-ZEB1-AS1+anti-miR-224 group showed increased OD values,the number of migrated and invaded cells,and expression levels of CyclinD1,MMP-2,and MMP-9 proteins(P＜0.05).Conclusion Inhibition of ZEB1-AS1 to targetedly upregulate of miR-224 expression can effectively reduce lymphoma cell prolifera-tion,invasion,and migration abilities.关键词
长链非编码RNA ZEB1反义RNA1/淋巴瘤/微小RNA-224/增殖/迁移/侵袭Key words
long non-coding RNA ZEB1-antisense RNA1/lymphoma/microRNA-224/pro-liferation/migration/invasion分类
医药卫生引用本文复制引用
施丹,沈超..长链非编码RNA ZEB1反义RNA1通过靶向微小RNA-224调控淋巴瘤细胞增殖、侵袭、迁移的分子机制研究[J].实用临床医药杂志,2024,28(22):35-40,6.基金项目
上海市崇明区"可持续发展科技创新行动计划"项目(CKY2022-20) （CKY2022-20）
