军事医学2024，Vol.48Issue(11)：832-837,6.DOI:10.7644/j.issn.1674-9960.2024.11.005
基于HPLC指纹图谱结合化学计量学方法分析评价鸡血藤超微粉的质量
Quality evaluation of ultrafine powder of Spatholobus suberectus based on HPLC fingerprint combined with stoichiometry methods
摘要
Abstract
Objective To evaluate the quality of ultrafine powder of Spatholobus suberectus from different sources by combined HPLC fingerprint and chemical pattern recognition.Methods Separation was performed on a Amethyst C18-H column(4.6 mmx250 mm,5 pm)and the mobile phase was 0.1％phosphoric acid-acetonitrile with gradient elution.The flow rate was 1.0 mL/min and the wavelength was 260 nm.The column temperature was 30 ℃.The cluster analysis(CA),least partial squares-discriminant analysis(PLS-DA)and principal component analysis(PCA)were applied to studying the HPLC fingerprint and chemical pattern recognition.Results Thirteen common peaks were identified in ultrafine powder samples of 10 batches of Spatholobus suberectus and 3 components were identified by reference substances.The similarity of fingerprint was 0.921-0.989,indicating good similarity.The samples were divided into 3 groups by cluster analysis;principal component analysis results extracted 4 principal components.According to the composite score,the quality of ultrafine powder samples of Spatholobus suberectus from S6,S1,S2,S3 were better than others.And 4 kinds of marker compounds that caused the quality difference of ultrafine powder samples of Spatholobus suberectu were screened out through the least partial squares-discriminant analysis,which were epicatechin and peaks 1,6 and 4 respectively.Conclusion The fingerprint method is simple and reproducible,and can be used to evaluate the quality of ultrafine powder samples of Spatholobus suberectus in combination with multi-mode stoichiometric analysis.关键词
鸡血藤/超微粉/指纹图谱/聚类分析/主成分分析/偏最小二乘法-判别分析Key words
Spatholobus suberectus/ultrafine powder/fingerprint/cluster analysis/principal component analysis/least partial squares-discriminant analysis分类
医药卫生引用本文复制引用
吴国学,杨书婷,刘栋,李小霞,董靖,郝义彬,王一硕..基于HPLC指纹图谱结合化学计量学方法分析评价鸡血藤超微粉的质量[J].军事医学,2024,48(11):832-837,6.基金项目
河南省中医药文化与管理研究项目重点课题(TCM2021002,TCM2023031) （TCM2021002,TCM2023031）
河南省医学科技攻关计划项目(20180208034,LHGJ20191089) （20180208034,LHGJ20191089）
河南省中医药科学研究专项课题(20-21ZY2141) （20-21ZY2141）
国家中医临床研究基地科研专项课题(2019JDZX2123) （2019JDZX2123）
