菌物研究2024，Vol.22Issue(4)：342-349,8.DOI:10.13341/j.jfr.2024.1780
灰盖粉褶鹅膏毒素检测及环肽基因家族分析
Detection of Amanita toxin(s)and Analysis of Cyclic Peptide Gene Family in Amanita griseorosea
摘要
Abstract
Amanita griseorosea is a lethal Amanita species within the sect.Phalloideae,mainly distributing in South China.In this study,toxins were detected through the methanol extraction and LC-MS analysis.Results showed that only α-amanitin was measured from this species,revealing an average concentration of(2 815.70±835.93)μg/g.In addition,genomic fragments from the MSDIN gene family were amplified by PCR for cloning and sequencing using degenerate primers.Seven MSDIN genes encoding different cyclic peptides were obtained.Sequence comparison and phylogenetic analysis indicated that one encodes the α-amanitin and another one encodes a cyclic peptide as previous reported.The remaining five all encode novel cyclic peptides.The above results thereafter provide an important foundation for further deep understanding of the toxicity and the capacity of cyclic peptide biosynthesis as well as the exploration of new cyclic peptides in A.griseorosea.关键词
鹅膏属/剧毒蘑菇/环肽毒素/环肽基因/聚类分析Key words
Amanita/poisonous mushroom/cyclic peptide toxin/cyclic peptide gene/phylogenetic analysis分类
农业科技引用本文复制引用
张成花,刘萌,王刚正,李泰辉,邓旺秋..灰盖粉褶鹅膏毒素检测及环肽基因家族分析[J].菌物研究,2024,22(4):342-349,8.基金项目
国家自然科学基金项目(32170009),国家重点研发计划项目(2021YFA0910200),科技部高端外国专家引进计划项目(G2022030055L) （32170009）
