菌物研究2024，Vol.22Issue(4)：378-386,9.DOI:10.13341/j.jfr.2023.1642
响应面法优化灵芝多糖提取工艺及其抗氧化活性
Optimization of Extraction Technology and Antioxidant Activity of Ganoderma lingzhi by Response Surface Method
摘要
Abstract
Response surface analysis was used to optimize the extraction process of Ganoderma lingzhi polysaccharides for the improvement of extraction rates and study of their antioxidant activity.Effects of methods,used time,solid-liquid ratio and number of times for extraction on polysaccharide yields were investigated.The results showed that reflux extraction method had the best extraction rate under the mostly suitable conditions as indicated below:temperature 85℃,time 4.2 h,solid-liquid ratio 1∶31 and twice extractions,which revealed the extraction rate of 1.676％.At the concentration of 1 mg/mL of G.lingzhi fruit body polysaccharides,the scavenging rates of DPPH free radical and hydroxyl-free radical were 94.23％and 61.21％,respectively.Our study thereafter provides a basic reference for the effective extraction of G.lingzhi polysaccharides and the development and utilization of their resources in future.关键词
灵芝/回流提取法/响应面法/多糖/抗氧化活性Key words
Ganoderma lingzhi/reflux extraction/response surface method/polysaccharide/anti-oxidant activity分类
农业科技引用本文复制引用
颜瑞辰,洪允炬,李悦,李长田,陈春宇,张影,华霜..响应面法优化灵芝多糖提取工艺及其抗氧化活性[J].菌物研究,2024,22(4):378-386,9.基金项目
吉林省教育厅项目(JJKH20220395KJ),吉林农业科技学院博士人才基金项目(111022022020),三峡库区道地药材开发利用重庆市重点实验室项目(Sys20210014),天津市合成生物技术创新能力提升行动项目(TSBICIP-CXRC-006) （JJKH20220395KJ）
