兰州大学学报（医学版）2024，Vol.50Issue(11)：8-16,9.DOI:10.13885/j.issn.1000-2812.2024.11.002
分级醇沉败酱草多糖的理化特征及抑制糖消化酶活性研究
Physicochemical characteristics of graded alcohol precipitation Herba Patriniapolisa Calid and inhibition of sugar-digesting enzyme activity
摘要
Abstract
Objective To investigate the physicochemical characteristics and glycodigestive enzyme inhibitory activity of different fractions of Herba patriniae polysaccharides,as well as the correlation between them.Methods Four kinds of HPs(HP-50,HP-60,HP-70 and HP-80)were obtained by hot water extraction and alcohol precipitation at different concentrations of ethanol(50%,60%,70%,80%),and the content and mono-saccharide composition of polysaccharides determined by phenol-sulfuric acid method,gas chromatography(GC),high performance liquid chromatography(GPC)and ultraviolet spectrophotometry(UV).The activities of four polysaccharides in inhibiting sugar-digesting enzymes were compared via a bivariate correlation analy-sis regarding their physical and chemical characteristics such as molecular weight and inhibitory activity against α-amylase and α-glucosidase.Results All HPs exhibited concentration-dependent inhibition of sugar-digesting enzymes,and HP-80 had the best inhibitory activity of sugar-digesting enzymes.Compared with oth-er alcohol precipitation concentrations,HP-80 had a higher glucose content,rhamnose and arabinose content was lower,and the molecular weight smaller.Conclusion It is speculated that the relative molecular weight of Herba patriniae polysaccharides is 50～100 kDa,the molar fraction of rhamnose less than 9.83%and the amount ratio of arabinose to glucose substance less than 1,which could more conducive to the inhibition of α-amylase and α-glucoside activities of HPs.关键词
分级醇沉/败酱草多糖/α-淀粉酶抑制活性/α-葡萄糖苷酶抑制活性/相关性分析Key words
graded alcohol sinking/Herba Patriniae polysaccharide/α-amylase inhibitory activity/α-glucosi dase inhibitory activity/correlation analysis分类
医药卫生引用本文复制引用
魏赛雪,惠和平,郭栋费,张心驰,郭敏,李晓东..分级醇沉败酱草多糖的理化特征及抑制糖消化酶活性研究[J].兰州大学学报（医学版）,2024,50(11):8-16,9.基金项目
甘肃省高等学校创新基金资助项目(2021A-260) （2021A-260）
国家自然科学基金资助项目(82160818) （82160818）
甘肃省中医药管理局科研资助项目(GZKP-2023-3) （GZKP-2023-3）
