山东科学2024，Vol.37Issue(6)：51-58,8.DOI:10.3976/j.issn.1002-4026.20240034
基于PI3K/AKT/Gsk-3β通路研究山药多糖对运动疲劳小鼠的影响
Effects of Dioscorea opposita Thunb.polysacchariades on exercise-related fatigue in mice through the PI3K/AKT/Gsk-3β pathway
摘要
Abstract
This study aimed to investigate the antifatigue effects and mechanisms of Dioscorea opposita Thunb.polysaccharides(DTPs)on exercise-related fatigue in mice.In a mouse model of exercise fatigue where mice were subjected to exhaustive swimming and fatigue rotarod,changes in exhaustive swimming duration,body weight,fatigue metabolite accumulation,activity levels of related enzymes,liver tissue structure,and muscle glycogen(MuG)and liver glycogen(LG)contents were assessed.The results demonstrated that DTPs significantly prolonged the exhaustive swimming duration and reduced body weight loss,hepatic damage,and serum lactic acid(LD),blood urea nitrogen(BUN),and lactate dehydrogenase(LDH)levels in the mice.DTPs also significantly enhanced superoxide dismutase(SOD)activity and MuG and LG contents and significantly reduced aspartate aminotransferase(AST)levels.Additionally,in mice administered DTPs,the mRNA expression of phosphoinositide 3-kinase(PI3K)was significantly upregulated,whereas that of protein kinase B(AKT)and glycogen synthase kinase-3(Gsk-3β)was significantly downregulated in skeletal muscle.Taken together,DTPs can reduce metabolite accumulation and ameliorate exercise fatigue by regulating the PI3K/AKT/Gsk-3β pathway to promote glycogen synthesis.关键词
山药多糖/抗运动疲劳/力竭游泳/PI3K/AKT/Gsk-3β通路Key words
Dioscorea opposita Thunb.polysaccharides/anti-exercise fatigue/exhaustive swimming/PI3K/AKT/Gsk-3β pathway分类
药学引用本文复制引用
曾婷,杨然,李庆军,宫亚楠,王书珍,刘谦..基于PI3K/AKT/Gsk-3β通路研究山药多糖对运动疲劳小鼠的影响[J].山东科学,2024,37(6):51-58,8.基金项目
山东省重点研发计划项目(2021CXGC010510) （2021CXGC010510）
财政部和农业农村部:国家现代农业产业技术体系项目(CARS-21) （CARS-21）
国家自然科学基金项目(82003892) （82003892）
泰山学者工程专项经费资助项目(tsqn202306187) （tsqn202306187）
中央引导地方科技发展资金项目(XZ202301YD0023C) （XZ202301YD0023C）
