蛹虫草提取物对高尿酸血症大鼠的降尿酸作用及机制OA北大核心CSTPCD

[Objective]To investigate the effect and the corresponding mechanism of Cordyceps militaris(C.militaris)extract on lowering uric acid by hyperuricemia model rats.[Method]The contents of total sugars,reducing sugars,polysaccharides,proteins and polyphenols in C.militaris extract were determined.The rat model of hyperuricemia was established based on feeding of potassium oxonate combined with yeast paste.The serum uric acid level,renal function,oxidative stress and inflammation of rats and the changes of intestinal microbial composition were measured to evaluate the effect of C.militaris extract on hyperuricemia rats and its mechanism.[Result]The C.militaris extract contained 35.86%polysaccharides,27.05%proteins,and 0.21%polyphenols.Animal experiments demonstrated that C.militaris extract significantly reduced the serum uric acid levels in hyperuricemia rats.The most effective dose was found to be 0.5 g/(kg·d),leading to a reduction in uric acid levels from 281.62 μmol/L to 93.27 μmol/L,with an inhibition rate of 66.88%.It also inhibited the oxidative stress and inflammation in renal tissue of hyperuricemia rats.Molecular mechanism studies showed that C.militaris extract down-regulated uric acid transporter1(URAT1)and glucose transporter 9(GLUT9),up-regulated the expressions of organic anion transporter 1(OAT1)and ATP binding cassette subfamily G member 2(ABCG2)in renal tissues of hyperuricemia rats.Concurrently,C.militaris extract significantly inhibited the xanthine oxidase activity in liver tissues.In addition,C.militaris extract enhanced the diversity of intestinal microbiota and maintained the balance of intestinal microecology in hyperuricemia rats.[Conclusion]C.militaris extract demonstrated a good anti-hyperuricemia effect,which might be related to regulating the expression of uric acid transporter and inhibiting the activity of xanthine oxidase.